Pseudomonas aeruginosa pyocyanin and 1-hydroxyphenazine inhibit fungal growth
Pseudomonas aeruginosa pyocyanin and 1-hydroxyphenazine inhibit fungal growth
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DOI：
10.1136/jcp.52.5.385
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发表时间：
1999-05-01
影响因子：
3.4
通讯作者：
Wilson, R
中科院分区：
文献类型：
--
作者：
Kerr, JR;Taylor, GW;Wilson, R
Aim-To examined strains of Pseudomonas aeruginosa for specific anti真菌factors. Methods-Two clinical strains of P aeruginosa with strong in vitro inhibition（by cross streak assay）of Candida albicans and Aspergillus fumigatus were examined.这两种菌株都是从肺结核中分离出来的，一种来自囊性纤维化患者，另一种来自支气管扩张患者。细菌提取物通过高效液相色谱法进行分级，并通过紫外吸收和质谱法进行检查。结果绿脓菌素是铜绿假单胞菌的主要抗真菌剂，1-羟基吩嗪也具有一定的抗真菌活性。绿脓菌素对白色念珠菌和烟曲霉的MIC> 64 μ g/ml。这些吩嗪对其他9种酵母菌的致病man. Preliminary实验也表明可能抑制酵母菌菌丝体转化在白色念珠菌绿脓菌素。结论绿脓菌素和1-羟基吩嗪可能是一个角色，在预防肺念珠菌病的患者定植铜绿假单胞菌。
Aim-To examine strains of Pseudomonas aeruginosa for specific antifungal factors.Methods-Two clinical strains of P aeruginosa with strong in vitro inhibition (by cross streak assay) of Candida albicans and Aspergillus fumigatus were examined. Both strains were isolated from sputum-one from a patient with cystic fibrosis and one from a patient with bronchiectasis. Bacterial extracts were fractionated by high performance liquid chromatography and examined by ultraviolet absorbance and mass spectroscopy. Antifungal activity against C albicans and A fumigatus was determined in a well plate assay.Results-Pyocyanin was the major antifungal agent of P aeruginosa; 1-hydroxyphenazine also possessed activity. Pyocyanin MICs for C albicans and A fumigatus were > 64 mu g/ml. These phenazines were active against nine other yeast species pathogenic for man. Preliminary experiments also suggested possible inhibition of yeast mycelial transformation in C albicans by pyocyanin.Conclusions-There may be a role for pyocyanin and 1-hydroxyphenazine in the prevention of pulmonary candidiasis in patients colonised by P aeruginosa.