A microfluidic platform for the investigation of elongation growth in pollen tubes
A microfluidic platform for the investigation of elongation growth in pollen tubes
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DOI：
10.1088/0960-1317/22/11/115009
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发表时间：
2012-11-01
影响因子：
2.3
通讯作者：
Geitmann, A.
中科院分区：
文献类型：
--
作者：
Agudelo, C. G.;Sanati, A.;Geitmann, A.
比率非常高，并且很容易在体外培养。花粉管生长研究的一个主要限制是难以提供物理上类似于体内条件的体外测试环境。这项工作展示了用于研究和操纵单个花粉管的微流体平台的开发。该平台采用硅/SU-8模具由聚二甲基硅氧烷制成，并利用微流体将花粉粒分配到连续排列的微通道中，管子在微通道中生长以允许进行单独测试。进行二维有限元流体分析以协助优化建筑设计。该装置的验证是通过种植山茶花粉来进行的。结果表明，微流体网络内的花粉管萌发和生长速率与传统平板或批量测定中获得的结果相似。微流体网络允许对各种结构特征进行特定测试，如简单的碰撞测试所示，并且它允许直接集成进一步的单细胞测试分析。
very high rates and are easily cultured in vitro. One major constraint in the study of pollen tube growth has been the difficulty in providing an in vitro testing environment that physically resembles the in vivo conditions. This work presents the development of a microfluidic platform for the study and manipulation of individual pollen tubes. The platform is fabricated from polydimethylsiloxane using a Silicon/SU-8 mold and makes use of microfluidics to distribute pollen grains to serially arranged microchannels into which the tubes grow to allow for individual testing. A 2D finite element fluid analysis is done to assist optimization of the architectural design. Validation of the device is carried out by growing Camellia japonica pollen. Results show that pollen tube germination and growth rate within the microfluidic network are similar to those obtained in conventional plate or batch assays. The microfluidic network allows for specific testing of a variety of structural features as demonstrated with a simple collision test, and it permits the straightforward integration of further single-cell test assays.