Granulocytes and cultured human fibroblasts express common acute lymphoblastic leukemia-associated antigens
Granulocytes and cultured human fibroblasts express common acute lymphoblastic leukemia-associated antigens
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粒细胞和培养的人成纤维细胞表达常见的急性淋巴细胞白血病相关抗原
DOI：
10.1182/blood.v61.4.718.bloodjournal614718
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发表时间：
1983
期刊：
影响因子：
20.3
通讯作者：
J. Hansen
中科院分区：
文献类型：
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作者：
MP Braun;P. Martin;J. Ledbetter;J. Hansen
我们已经研究了常见的急性淋巴细胞白血病抗原（cALLA）的非淋巴细胞的表达，使用一种新的鼠单克隆抗体（指定24.1），特异性的cALLA。该抗体完全阻断单克隆抗cALLA抗体J-5的结合。此外，与抗体J-5相比，抗体24.1以更大的亲和力结合cALLA并诱导更大程度的抗原调节。用抗体24.1，我们已经证明正常培养的骨髓和皮肤成纤维细胞和成熟粒细胞表达cALLA。通过免疫沉淀和聚丙烯酰胺凝胶电泳，来自成纤维细胞的cALLA具有比来自培养的人白血病细胞系的cALLA略低的分子量，而来自粒细胞的cALLA具有比来自培养的人白血病细胞系的cALLA略高的分子量。然而，定量研究表明，正常粒细胞和皮肤成纤维细胞上的cALLA表达分别比NALM-6细胞低约3倍和20倍。cALLA可由在多个器官系统中广泛分布的细胞表达。这些发现强调，分化标志物，似乎是肿瘤或组织特异性可能会发现不同来源的细胞。
We have investigated the expression of the common acute lymphoblastic leukemia antigen (cALLA) by nonlymphoid cells, using a new murine monoclonal antibody (designated 24.1), specific for cALLA. This antibody completely blocks the binding of monoclonal anti-cALLA antibody J-5. Furthermore, antibody 24.1 binds to cALLA with greater affinity and induces a greater degree of antigenic modulation than antibody J-5. With antibody 24.1, we have demonstrated that normal cultured marrow and skin fibroblasts and mature granulocytes express cALLA. By immune precipitation and polyacrylamide gel electrophoresis, cALLA from fibroblasts has a slightly lower molecular weight and cALLA from granulocytes a slightly higher molecular weight than cALLA from cultured human leukemic cell lines. Quantitative studies indicate, however, that cALLA expression is approximately 3-fold and 20-fold lower, respectively, on normal granulocytes and skin fibroblasts than on NALM-6 cells. cALLA may be expressed by cells with widespread distribution in multiple organ systems. These findings emphasize that differentiation markers that appear to be tumor or tissue specific may be found on cells of diverse origin.
抗体亲和力可能会影响体外常见急性淋巴细胞白血病抗原的抗原调节。
DOI：
--
发表时间：
1982
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Lebien,TW;Boue,DR;Bradley,JG;Kersey,JH
通讯作者：
Kersey,JH
单克隆抗体体外诱导人急性淋巴细胞白血病抗原的调节。
DOI：
--
发表时间：
1980
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Ritz,J;Pesando,JM;Notis-McConarty,J;Schlossman,SF
通讯作者：
Schlossman,SF