Three-dimensional fluorescence-enhanced optical tomography using a hand-held probe based imaging system
Three-dimensional fluorescence-enhanced optical tomography using a hand-held probe based imaging system
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DOI：
10.1118/1.2940603
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发表时间：
2008-07-01
期刊：
影响因子：
3.8
通讯作者：
Godavarty, Anuradha
中科院分区：
文献类型：
--
作者：
Ge, Jiajia;Zhu, Banghe;Godavarty, Anuradha
基于手持的光学成像系统是朝向乳腺癌的诊断成像的最新发展。到目前为止，所有的手持光学成像仪仅用于执行表面映射和目标定位，但不能演示断层成像。在此，一种新型的手持探头为基础的光学成像仪开发的三维（3-D）光学断层扫描研究。该光学成像仪主要由手持式探头和增强型电荷耦合器件检测器组成，其独特功能是能够：（i）对大组织区域（5x 10平方米）成像，cm），（ii）同时执行多点照明和收集，从而减少总体成像时间;以及（iii）从灵活的探头设计适应变化的组织曲率。实验研究在频域中在大平板体模（类似于650 ml）上进行，使用在完美摄取（1：0）对比度下的荧光靶，以及变化的靶深度（1-2 cm）和X-Y位置。实验证明了同时多点照明对三维层析成像的影响。三维光学层析成像研究的可行性已被证明首次使用手持光学成像仪。初步的荧光增强光学断层扫描研究能够重建位于不同目标深度（1-2 cm）的0.45 ml目标。然而，随着实际目标深度的增加，深度恢复受到限制，因为仅获得反射率测量值。目前正在进行广泛的断层扫描研究，以确定成像仪在平面和曲面体模上的分辨率和性能极限。(C)2008年美国医学物理学家协会。
Hand-held based optical imaging systems are a recent development towards diagnostic imaging of breast cancer. To date, all the hand-held based optical imagers are used to perform only surface mapping and target localization, but are not capable of demonstrating tomographic imaging. Herein, a novel hand-held probe based optical imager is developed towards three-dimensional (3-D) optical tomography studies. The unique features of this optical imager, which primarily consists of a hand-held probe and an intensified charge coupled device detector, are its ability to; (i) image large tissue areas (5x10 sq. cm) in a single scan, (ii) perform simultaneous multiple point illumination and collection, thus reducing the overall imaging time; and (iii) adapt to varying tissue curvatures, from a flexible probe head design. Experimental studies are performed in the frequency domain on large slab phantoms (similar to 650 ml) using fluorescence target(s) under perfect uptake (1:0) contrast ratios, and varying target depths (1-2 cm) and X-Y locations. The effect of implementing simultaneous over sequential multiple point illumination towards 3-D tomography is experimentally demonstrated. The feasibility of 3-D optical tomography studies has been demonstrated for the first time using a hand-held based optical imager. Preliminary fluorescence-enhanced optical tomography studies are able to reconstruct 0.45 ml target(s) located at different target depths (1-2 cm). However, the depth recovery was limited as the actual target depth increased, since only reflectance measurements were acquired. Extensive tomography studies are currently carried out to determine the resolution and performance limits of the imager on flat and curved phantoms. (C) 2008 American Association of Physicists in Medicine.