Monitoring of Atg5-Independent Mitophagy

Monitoring of Atg5-Independent Mitophagy
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Atg5 独立线粒体自噬的监测

DOI:
10.1007/7651_2017_16
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发表时间:
2017
期刊:
Methods Mol Biol.
影响因子:
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通讯作者:
Shimizu Shigeomi
Shimizu Shigeomi
中科院分区:
--
文献类型:
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作者:
Arakawa Satoko;Honda Shinya;Torii Satoru;Tsujioka Masatsune;Shimizu Shigeomi

文献摘要

相似文献

线粒体自噬是一种线粒体质量控制机制,其中受损和多余的线粒体通过自噬去除。至少有两种不同的哺乳动物自噬途径:依赖atg5的常规途径和不依赖atg5的替代途径;后者参与红细胞有丝分裂。在本章中,我们描述了各种实验方法来评估哺乳动物细胞中atg5独立的有丝自噬。
Mitophagy is a mitochondrial quality control mechanism where damaged and surplus mitochondria are removed by autophagy. There are at least two different mammalian autophagy pathways: the Atg5-dependent conventional pathway and an Atg5-independent alternative pathway; the latter is involved in the erythrocyte mitophagy. In this chapter we describe the various experimental approaches to assess Atg5-indepedndent mitophagy in mammalian cells.