An R-selective hydroxynitrile lyase from Arabidopsis thaliana with an α/β-hydrolase fold
An R-selective hydroxynitrile lyase from Arabidopsis thaliana with an α/β-hydrolase fold
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DOI：
10.1002/anie.200701455
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发表时间：
2007-01-01
影响因子：
16.6
通讯作者：
Pohl, Martina
中科院分区：
文献类型：
--
作者：
Andexer, Jennifer;von Langermann, Jan;Pohl, Martina
1. Cloning and expression of AtHNL The AtHNL-gene was amplified from Arabidopsis cDNA by polymerase chain reaction (PCR). Specific primers were derived from the sequence in the database (gene bank entry AAN13041) including appropriate restriction sites for cloning of the gene into the vector pET28a (Novagen).5’-primer: TATACCATGGAGAGGAAACATCACTTCGTGTTAGTTCACA NcoI site3’-primer: TATACTCGAGTTACATATAATCGGTGGCAATAGCAGAGAG XhoI site
