Replication-competent retroviral vectors for expressing genes in avian cells in vitro and in vivo
Replication-competent retroviral vectors for expressing genes in avian cells in vitro and in vivo
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DOI：
10.1007/bf02740819
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发表时间：
1997-06-01
影响因子：
2.6
通讯作者：
Brickell, PM
中科院分区：
文献类型：
--
作者：
Bell, EJD;Brickell, PM
基于Rous肉瘤病毒(RSV)的可复制逆转录病毒载体在原代细胞培养和鸡卵胚胎组织中表达基因正变得越来越受欢迎。在这篇文章中，我们综述了RSV的特性及其适合用作载体的生命周期。我们描述了RCA和RCAS(BP)系列载体的设计和使用，它们是目前使用最广泛的基于RSV的载体，说明了它们的优点和缺点。最后，我们概述了适用于处理这些逆转录病毒载体的实验室方案。
Replication-competent retroviral vectors based on Rous sarcoma virus (RSV) are becoming increasingly popular for expressing genes in both primary cell cultures and embryonic chick tissues in ovo. In this article we review the features of RSV and its life cycle that make it suitable for use as a vector. We describe the design and use of the RCA and RCAS(BP) series of vectors which are currently the most widely used RSV-based vectors, illustrating both their strengths and weaknesses. Finally, we outline laboratory protocols suitable for the handling of these retroviral vectors.