CHARACTERIZATION OF 2 DISTINCT MONOCLONAL-ANTIBODIES TO PAIRED HELICAL FILAMENTS - FURTHER EVIDENCE FOR FETAL-TYPE PHOSPHORYLATION OF THE TAU IN PAIRED HELICAL FILAMENTS
CHARACTERIZATION OF 2 DISTINCT MONOCLONAL-ANTIBODIES TO PAIRED HELICAL FILAMENTS - FURTHER EVIDENCE FOR FETAL-TYPE PHOSPHORYLATION OF THE TAU IN PAIRED HELICAL FILAMENTS
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DOI：
10.1111/j.1471-4159.1993.tb03491.x
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发表时间：
1993-06-01
影响因子：
4.7
通讯作者：
IHARA, Y
中科院分区：
文献类型：
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作者：
HASEGAWA, M;WATANABE, A;IHARA, Y
两个单克隆抗体C5和M4提出了对Sarkosyl不溶性配对螺旋丝（PHF）特异性标记胎儿tau，但几乎不标记正常成人tau。C5免疫反应性通过碱性磷酸酶在37 ℃下处理而消除，而M4反应性仅可通过在67 ℃下处理而消除。表位分析表明，C5和M4识别位点分别在残基386-406和198-250中，根据最长的人tau同种型的编号。因此，磷酸化位点位于微管结合区的氨基和羧基末端部分。这两种充分表征的单克隆抗体在鉴定将正常tau转化为PHF-tau的蛋白激酶中应该是有价值的。
Two monoclonal antibodies C5 and M4 raised against Sarkosyl-insoluble paired helical filaments (PHF) specifically labeled fetal tau, but hardly labeled normal adult tau. C5 immunoreactivity was eliminated by alkaline phosphatase treatment at 37-degrees-C, whereas M4 reactivity could be removed only by the treatment at 67-degrees-C. Epitope analysis showed that C5 and M4 recognition sites are in residues 386-406 and 198-250, respectively, according to the numbering of the longest human tau isoform. Thus, the phosphorylation sites are located in the amino- and carboxyl-terminal portions of the microtubule-binding region. These two well-characterized monoclonals should be valuable in the identification of a protein kinase(s) that converts normal tau into PHF-tau.