REFOLDING AND ORIENTED INSERTION OF A MEMBRANE-PROTEIN INTO A LIPID BILAYER
REFOLDING AND ORIENTED INSERTION OF A MEMBRANE-PROTEIN INTO A LIPID BILAYER
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DOI：
10.1073/pnas.89.16.7457
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发表时间：
1992-08-15
影响因子：
11.1
通讯作者：
JAHNIG, F
中科院分区：
文献类型：
--
作者：
SURREY, T;JAHNIG, F
我们研究了大肠杆菌外膜蛋白OmpA的复性和膜插入。在尿素存在下通过超声处理从其天然膜中提取蛋白质，并以未折叠形式溶解在尿素/水混合物中。在这种形式下，它被纯化了。在添加预先形成的脂质囊泡后，蛋白质自发地重折叠并插入囊泡膜中。囊泡必须很小，脂质必须处于流体状态。以定向方式插入。
We have studied the refolding and membrane insertion of the outer membrane protein OmpA of Escherichia coli. The protein was extracted from its native membrane by sonication in the presence of urea and dissolved in the urea/water mixture in unfolded form. In this form it was purified. Upon addition of preformed lipid vesicles, the protein spontaneously refolded and inserted into the vesicle membranes. The vesicles had to be small and the lipids had to be in the fluid state. The insertion occurred in an oriented manner.