Transgene analysis proves mRNA trans-splicing at the complex mod(mdg4) locus Drosophila
Transgene analysis proves mRNA trans-splicing at the complex mod(mdg4) locus Drosophila
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DOI：
10.1073/pnas.151268698
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发表时间：
2001-08-14
影响因子：
11.1
通讯作者：
Loewendorf, A
中科院分区：
文献类型：
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作者：
Dorn, R;Reuter, G;Loewendorf, A
含有基因MOD的果蝇BTB结构域（MDG4）产生大量蛋白质同工型，将402 AA的常见N末端区域与不同的C末端组合在一起。我们推断了该复合物基因座的基因组结构，发现至少有七个MOD（MDG4）同工型在其两个反平行DNA链上编码，这表明通过移植分裂产生了成熟的mRNA。在转基因测定中，我们证明了果蝇通过从插入远处染色体位置插入的转基因产生的前MRNA进行的跨跨基因来产生MOD（MDG4）mRNA的能力。此外，有证据表明发生了由平行DNA链编码的MOD（MDG4）特异性外显子的变形。 MOD（MDG4）基因座代表了一种新型的COMLPEX基因结构，其中通过广泛的移媒体进行了遗传复杂性，从而对基因组测序项目产生了重要意义。在模型有机体中，果蝇模型中天然发生的变形的证明开辟了新的实验方法，以分析潜在机制。
The Drosophila BTB domain containing gene mod(mdg4) produces a large number of protein isoforms combining a common N-terminal region of 402 aa with different C termini. We have deduced the genomic structure of this complex locus and found that at least seven of the mod(mdg4) isoforms are encoded on both of its antiparallel DNA strands, suggesting the generation of mature mRNAs by trans-splicing. In transgenic assays, we demonstrate the ability of Drosophila to produce mod(mdg4) mRNAs by trans-splicing of pre-mRNAs generated from transgenes inserted at distant chromosomal positions. Furthermore, evidence is presented for occurring of trans-splicing of mod(mdg4)-specific exons encoded by the parallel DNA strand. The mod(mdg4) locus represents a new type of comlpex gene structure in which genetic complexity is resolved by extensive trans-splicing, giving important implications for genome sequencing projects. Demonstration of naturally occurring trans-splicing in the model organism Drosophila opens new experimental approaches toward an analysis of the underlying mechanisms.