Activated B cells express receptors for, and proliferate in response to, pure interleukin 2
Activated B cells express receptors for, and proliferate in response to, pure interleukin 2
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激活的 B 细胞表达纯白细胞介素 2 的受体，并响应纯白细胞介素 2 进行增殖
DOI：
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发表时间：
1984
影响因子：
15.3
通讯作者：
H. Macdonald
中科院分区：
文献类型：
--
作者：
R. Zubler;J. Lowenthal;F. Erard;N. Hashimoto;R. Devos;H. Macdonald
在这项研究中，我们研究了白细胞介素2（IL-2）是否对B细胞增殖的作用，以及活化的B细胞是否表达IL-2受体。首先，在B母细胞测定中使用已被脂多糖（LPS）加抗Ig抗体（抗Ig）激活的阳性选择的鼠表面Ig阳性细胞研究免疫亲和纯化或重组人IL-2的功能活性。在该测定中，通过荧光激活细胞分选仪分析未检测到T细胞。发现与从鼠或人脾细胞或鼠克隆的T辅助细胞获得的上清液相比，两种IL-2制剂导致最佳的B细胞增殖。第二，我们观察到，在该测定中的IL-2需求与使用凝集素活化的多克隆鼠Lyt-2阳性T细胞的增殖测定中的IL-2需求大致相同。第三，对放射性标记的免疫亲和纯化的IL-2与B细胞的结合的分析表明，LPS加抗Ig活化的B细胞平均表达3，500个IL-2受体/细胞，表观解离常数为150 pM。然而，无论是未活化的B细胞还是单独由LPS活化的B细胞都没有表现出显著的特异性IL-2结合。功能和受体数据与IL-2不仅是T细胞而且是B细胞的生长因子的结论一致。
In this study we investigated whether interleukin 2 (IL-2) acts on B cell proliferation and whether activated B cells express IL-2 receptors. First, the functional activity of immunoaffinity-purified or recombinant human IL-2 was studied in a B blast assay using positively selected murine surface Ig-positive cells that had been activated by lipopolysaccharide (LPS) plus anti-Ig antibodies (anti-Ig). In this assay, T cells were not detected by fluorescence-activated cell sorter analysis. It was found that both IL-2 preparations led to optimal B cell proliferation compared with supernatants obtained from murine or human spleen cells or murine cloned T helper cells. Second, we observed that the IL-2 requirement in this assay was about the same as in a proliferation assay using lectin-activated polyclonal murine Lyt-2- positive T cells. Third, analysis of the binding of radiolabeled immunoaffinity-purified IL-2 to B cells indicated that LPS plus anti-Ig- activated B cells expressed a mean of 3,500 IL-2 receptors per cell with an apparent dissociation constant of 150 pM. However, neither nonactivated B cells nor B cells activated by LPS alone exhibited significant specific IL-2 binding. The functional and the receptor data are consistent with the conclusion that IL-2 is a growth factor not only for T cells but also for B cells.
抗体对“T 细胞非依赖性”抗原的反应需要非特异性 T 细胞因子。
DOI：
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发表时间：
1983
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Endres,RO;Kushnir,E;Kappler,JW;Marrack,P;Kinsky,SC
通讯作者：
Kinsky,SC
影响因子：
4.4
作者：
K. Smith;M. Favata;S. Oroszlan
通讯作者：
K. Smith;M. Favata;S. Oroszlan
影响因子：
4.4
作者：
T. Kakiuchi;R. Chesnut;H. Grey
通讯作者：
T. Kakiuchi;R. Chesnut;H. Grey