Evidence for a Mechanism of Recombination during Reverse Transcription Dependent on the Structure of the Acceptor RNA* 210
Evidence for a Mechanism of Recombination during Reverse Transcription Dependent on the Structure of the Acceptor RNA* 210
复制标题
逆转录过程中重组机制依赖于受体 RNA* 210 结构的证据
DOI：
--
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发表时间：
2003
影响因子：
4.8
通讯作者：
M. Negroni
中科院分区：
文献类型：
--
作者：
A. Moumen;Lucette Polomack;T. Unge;M. Véron;H. Buc;M. Negroni
基因重组是驱动逆转录病毒进化的主要力量。在逆转录病毒中，重组主要通过逆转录过程中的拷贝选择进行。使用重组的体外系统，我们已经研究了链转移的机制上的一个主要的重组热点，我们以前确定的基因组内的HIV-1。我们表明，在这个模型序列的复制选择的频率受到强烈的影响，折叠的RNA模板，即由一个稳定的发夹的存在。该结构必须特异性地存在于受体模板上。我们以前提出，链转移遵循两个步骤的过程：对接新生的DNA到受体RNA和链入侵。复制选择条件下的重组的频率是不依赖于受体RNA的浓度，相反，在强烈逮捕逆转录发生链转移。在拷贝选择链转移期间，对接步骤不是速率限制。我们提出，受体RNA上存在的发夹可以介导链转移的机制让人想起DNA重组过程中的分支迁移。
Genetic recombination is a major force driving retroviral evolution. In retroviruses, recombination proceeds mostly through copy choice during reverse transcription. Using a reconstituted in vitro system, we have studied the mechanism of strand transfer on a major recombination hot spot we previously identified within the genome of HIV-1. We show that on this model sequence the frequency of copy choice is strongly influenced by the folding of the RNA template, namely by the presence of a stable hairpin. This structure must be specifically present on the acceptor template. We previously proposed that strand transfer follows a two-step process: docking of the nascent DNA onto the acceptor RNA and strand invasion. The frequency of recombination under copy choice conditions was not dependent on the concentration of the acceptor RNA, in contrast with strand transfer occurring at strong arrests of reverse transcription. During copy choice strand transfer, the docking step is not rate limiting. We propose that the hairpin present on the acceptor RNA could mediate strand transfer following a mechanism reminiscent of branch migration during DNA recombination.
两种有缺陷的逆转录酶可以互补以恢复逆转录病毒的感染性。
DOI：
10.1002/j.1460-2075.1993.tb06128.x
发表时间：
1993
期刊：
The EMBO journal
影响因子：
--
作者：
Telesnitsky,A;Goff,SP
通讯作者：
Goff,SP
DOI：
10.1073/pnas.87.4.1556
发表时间：
1990-02-01
影响因子：
11.1
作者：
HU, WS;TEMIN, HM
通讯作者：
TEMIN, HM