Genome-Wide Analysis of the SWEET Transporters and Their Potential Role in Response to Cold Stress in Rosa rugosa
Genome-Wide Analysis of the SWEET Transporters and Their Potential Role in Response to Cold Stress in Rosa rugosa
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DOI：
10.3390/horticulturae9111212
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发表时间：
2023-11
期刊：
影响因子：
3.1
通讯作者：
Ronghui Li;Peng Gao;Tao Yang;Jie Dong;Yunting Chen;Yangyang Xie;Yvtong Yang;Chengzhi Liu-
中科院分区：
文献类型：
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作者：
Ronghui Li;Peng Gao;Tao Yang;Jie Dong;Yunting Chen;Yangyang Xie;Yvtong Yang;Chengzhi Liu-
糖最终输出转运蛋白（Sugar Will最终be export Transporter， SWEET）是最近发现的一组外排转运蛋白，在糖外排、韧皮部负荷、生殖组织发育和应激反应中起重要作用。迄今为止，还没有关于玫瑰（r.r ugosa） SWEET基因的报道。在本研究中，我们对SWEET基因进行了全面的基因组分析，包括染色体定位、系统发育比较、顺式调控元件分析、不同组织表达模式分析、冷胁迫下表达模式分析和亚细胞定位分析。共鉴定出33个rrsweet成员，并将其划分为4个不同的分支（Clade I、Clade II、Clade III和Clade IV）。它们分布在7条染色体上，含有与激素和应激反应相关的顺式调控元件。RrSWEETs的表达具有组织特异性，在根、花或雌蕊中的表达高于其他组织。此外，在整个冷胁迫过程中，RrSWEET4、16和20的相对表达量均显著上调，尤其是在红草根和茎中。亚细胞定位分析显示，RrSWEET4、16和20位于细胞膜上。综上所述，本研究结果为今后进一步研究RrSWEET基因在红壤中的功能及其在抗寒反应中的作用提供了理论基础。
Sugar Will Eventually be Exported Transporter (SWEET) proteins are a recently discovered group of efflux transporters that play essential roles in sugar efflux, phloem loading, reproductive tissue development and stress responses. To date, there have been no reports on the Rosa rugosa (R. rugosa) SWEET genes. In this study, we conducted a comprehensive genomic analysis of the SWEET genes, including chromosome localization, phylogenetic comparison, cis-regulatory element analysis, expression pattern analysis in different tissues, expression pattern analysis under cold stress and subcellular localization analysis. A total of 33RrSWEET members were identified and classified into four distinct clades (Clade I, Clade II, Clade III and Clade IV). They were distributed across seven chromosomes and contained cis-regulatory elements associated with hormone and stress responses. The expression of RrSWEETs showed tissue specificity, with higher expression in roots, flowers or pistils compared to other tissues. Furthermore, during the entire cold stress process, the relative expression levels of RrSWEET4, 16 and 20 were significantly upregulated, especially in the roots and stems of R. rugosa. Subcellular localization analysis revealed that RrSWEET4, 16 and 20 were located on the cell membrane. In summary, the results of this study provide a theoretical basis for future research on the functions of RrSWEET genes in R. rugosa and their role in cold tolerance responses.