PLASMODIUM-FALCIPARUM-SPECIFIC IMMUNOGLOBULIN-G (IGG), IGM, AND IGE ANTIBODIES IN PAIRED MATERNAL-CORD SERA FROM EAST SEPIK PROVINCE, PAPUA-NEW-GUINEA
PLASMODIUM-FALCIPARUM-SPECIFIC IMMUNOGLOBULIN-G (IGG), IGM, AND IGE ANTIBODIES IN PAIRED MATERNAL-CORD SERA FROM EAST SEPIK PROVINCE, PAPUA-NEW-GUINEA
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DOI：
10.1128/iai.61.3.988-993.1993
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发表时间：
1993-03-01
影响因子：
3.1
通讯作者：
ALPERS, MP
中科院分区：
文献类型：
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作者：
DESOWITZ, RS;ELM, J;ALPERS, MP
对来自东塞皮克（巴布亚新几内亚）产妇及其新生儿的46对母 - 脐血清进行了酶联免疫吸附测定（ELISA）和蛋白质印迹（免疫印迹）血清学分析，以检测针对恶性疟原虫的免疫球蛋白G（IgG）、IgM和IgE抗体。对这些妇女的同期研究表明，胎盘寄生虫血症发生率与产次有关，初产妇组发生率最高（41%），生育三次以上的妇女（>3产次组）发生率最低（3%）。母体血清中抗疟疾IgG、IgM和IgE抗体的总体ELISA阳性率分别为54.3%、28.2%和8.3%，而脐带血清中的阳性率分别为36.9%、0和16.6%。血清阳性率与产妇产次组无关，但母体IgE除外，>3产次组的阳性率较高，具有临界显著性，高于初产妇组。脐带IgE阳性在很大程度上与母体阳性无关，反之亦然。脐带和母体IgG免疫印迹对显示出近乎同源性。针对大小为……的恶性疟原虫抗原的IgG抗体
Enzyme-linked immunosorbent assay (ELISA) and Western blot (immunoblot) serological analyses for immunoglobulin G (IgG), IgM, and IgE antibodies to Plasmodium falciparum were made from 46 maternal-cord serum pairs obtained from parturient East Sepik (Papua New Guinea) women and their newborn. Concurrent study of these women had shown that placental parasitemia rates were related to parity with the highest rate (41%) in the primiparous group and the lowest rate (3%) in the women who had given birth more than three times (>3 parity group). Overall ELISA positivity rates for antimalarial IgG, IgM, and IgE antibodies in the maternal sera were 54.3, 28.2, and 8.3%, respectively, while those for the cord sera were 36.9, 0, and 16.6% respectively. Seropositivity rates were not related to maternal parity group, except for maternal IgE, in which there was a higher rate, of borderline significance, in the >3 parity group than in the primiparous group. Cord IgE positivity was largely independent of maternal positivity and vice versa. Cord and maternal IgG immunoblot pairs showed near homology. IgG antibodies to the P. falciparum antigens of sizes