SHORT-TERM DIETARY RESTRICTION IN OLD ZEBRAFISH CHANGES CELL SENESCENCE MECHANISMS
SHORT-TERM DIETARY RESTRICTION IN OLD ZEBRAFISH CHANGES CELL SENESCENCE MECHANISMS
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DOI：
10.1016/j.neuroscience.2016.07.033
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发表时间：
2016-10-15
期刊：
影响因子：
3.3
通讯作者：
Adams, Michelle M.
中科院分区：
文献类型：
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作者：
Arslan-Ergul, Ayca;Erbaba, Begun;Adams, Michelle M.
大脑老化的特点是认知能力下降，并与神经退行性疾病有关。最近的研究表明，神经发生持续到成年期，但已知在年龄增长期间会减少，这些变化可能有助于认知改变。旨在促进更好老龄化的进展至关重要。饮食限制（DR）是唯一可靠地延长寿命和健康寿命的非遗传干预措施。DR和年龄如何以及为什么影响神经发生的机制还没有很好地理解，并且在斑马鱼中没有得到太多的利用，由于广泛可用的遗传工具，斑马鱼已经成为研究脑老化和神经退行性疾病的流行模型。在这项研究中，我们使用年轻（8-8.5个月）和老年（26-32.5个月）斑马鱼作为模型，以研究短期DR对活跃增殖细胞的影响。我们成功地将10周的DR应用于年轻和年老的鱼，这导致两组鱼的体重显著减轻，对身体生长中与年龄相关的正常变化没有影响。我们发现，年龄降低细胞增殖和增加衰老相关的β-半乳糖苷酶，以及缩短端粒长度。相比之下，DR缩短端粒长度只在年轻的动物。年龄和DR均不改变胶质细胞的分化模式。我们的研究结果表明，DR的潜在影响可能是由端粒调节介导的，这些是有益的还是负面的仍有待确定。(C)2016年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Brain aging is marked by a decline in cognitive abilities and associated with neurodegenerative disorders. Recent studies have shown, neurogenesis continues into adulthood but is known to be decreasing during advancing age and these changes may contribute to cognitive alterations. Advances, which aim to promote better aging are of paramount importance. Dietary restriction (DR) is the only non-genetic intervention that reliably extends life and health-span. Mechanism's of how and why DR and age affect neurogenesis are not well-understood, and have not been utilized much in the zebrafish, which has become a popular model to study brain aging and neurodegenerative disease due to widely available genetic tools. In this study we used young (8-8.5 months) and old (26-32.5 months) zebrafish as the model to investigate the effects of a short-term DR on actively proliferating cells. We successfully applied a 10-week DR to young and old fish, which resulted in a significant loss of body weight in both groups with no effect on normal age-related changes in body growth. We found that age decreased cell proliferation and increased senescence associated beta-galactosidase, as well as shortened telomere lengths. In contrast, DR shortened telomere lengths only in young animals. Neither age nor DR changed the differentiation patterns of glial cells. Our results suggest that the potential effects of DR could be mediated by telomere regulation and whether these are beneficial or negative remains to be determined. (C) 2016 IBRO. Published by Elsevier Ltd. All rights reserved.