THE RATE OF DECAY OF RHODOBACTER-CAPSULATUS-SPECIFIC PUF MESSENGER-RNA SEGMENTS IS DIFFERENTIALLY AFFECTED BY RNASE-E ACTIVITY IN ESCHERICHIA-COLI
THE RATE OF DECAY OF RHODOBACTER-CAPSULATUS-SPECIFIC PUF MESSENGER-RNA SEGMENTS IS DIFFERENTIALLY AFFECTED BY RNASE-E ACTIVITY IN ESCHERICHIA-COLI
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DOI：
10.1016/0378-1119(92)90166-m
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发表时间：
1992-11-02
期刊：
影响因子：
3.5
通讯作者：
ROTHFUCHS, R
中科院分区：
文献类型：
--
作者：
KLUG, G;JOCK, S;ROTHFUCHS, R
在荚膜红细菌中，puf操纵子编码光合机构的蛋白质。多顺反子puf mRNA由显示差异稳定性的片段组成。在这里，我们表明，在大肠杆菌中的2.7-kb的pufBALMX mRNA种类的衰减率取决于核糖核酸酶E（RNase E）的活性，而0.5-kb的pufBA mRNA片段的降解不受me基因突变的影响。RNase E促进的pufLMX mRNA的降解依赖于1.4 kb pufLM mRNA片段的存在，其中速率限制性内切核酸裂解被假定发生在R.囊状的将该1.4kb片段中的185 bp插入pufB中，导致修饰的pufBA mRNA片段在E.杆菌我们的研究结果表明，在R。荚膜核糖核酸酶E样活性负责pufLM mRNA片段内发生的限速核酸内切裂解，而0.5-kb pufBA mRNA片段通过不同的RNA酶E-独立的衰变机制降解。
In Rhodobacter capsulatus the puf operon encodes proteins of the photosynthetic apparatus. The polycistronic puf mRNA is comprised of segments that show differential stability. Here, we show that the rate of decay of the 2.7-kb pufBALMX mRNA species in Escherichia coli depends on the activity of ribonuclease E (RNase E), whereas the degradation of the 0.5-kb pufBA mRNA segment is not affected by a mutation in the me gene. The RNase E-promoted decay of the pufLMX mRNA depends on the presence of a 1.4-kb pufLM mRNA segment, in which rate-limiting endonucleolytic cleavage was postulated to occur in R. capsulatus. The insertion of 185 bp of this 1.4-kb segment into pufB results in an RNase E-dependent decay of the modified pufBA mRNA segment in E. coli. Our findings suggest that in R. capsulatus an RNase E-like activity is responsible for the rate-limiting endonucleolytic cleavage occurring within the pufLM mRNA segment, whereas the 0.5-kb pufBA mRNA segment is degraded by a different RNase E-independent decay mechanism.