Study on Association of Pentraxin 3 and Diabetic Nephropathy in a Rat Model.
Study on Association of Pentraxin 3 and Diabetic Nephropathy in a Rat Model.
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DOI：
10.1155/2018/8968573
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发表时间：
2018
影响因子：
4.3
通讯作者：
Zhu H
中科院分区：
文献类型：
--
作者：
Chen X;Luo J;Wu M;Pan Z;Xie Y;Wang H;Chen B;Zhu H
糖尿病肾病（diabetic nephropathy，DN）是糖尿病严重的微血管并发症。与其他糖尿病治疗方法相比，胰岛移植可有效预防和逆转糖尿病视网膜病变、糖尿病肾病等微血管病变。PTX 3是唯一可以在肾组织中检测到的长五聚蛋白。在这项研究中，我们研究了胰岛移植后早期DN逆转时PTX 3的表达。方法.用链脲佐菌素（STZ）诱导大鼠糖尿病模型。12周后，将糖尿病大鼠分为胰岛移植组（IT）和糖尿病肾病组（DN）。分别采用尿常规、免疫组化和Western blot检测肾损伤、肾功能以及血浆和肾脏中PTX 3的表达。结果PTX3在DN组肾组织中的表达明显降低，而在IT组肾组织中的表达则因DN的逆转而明显升高。结论.我们的数据表明，肾组织中的PTX 3水平与DN的肾损伤密切相关。这可能用于量化DN中肾损伤的程度，提供早期DN患者中肾小管损伤的潜在早期指标，并评估DN临床进展。
Diabetic nephropathy (DN) is a serious microvascular complication of diabetes. Compared with other therapies for diabetic patients, islet transplantation can effectively prevent and reverse diabetes-induced microvascular disease, such as diabetic retinopathy and nephropathy. PTX3 is the only long pentraxin that can be detected in renal tissue. In this study, we investigated the expression of PTX3 when early DN was reversed after islet transplantation. Methods. Diabetes was induced in rats by injecting streptozotocin (STZ). Twelve weeks later, the diabetic rats were divided into 2 groups: the islet transplantation group (IT) and the diabetic nephropathy group (DN). Renal injury, renal function, and the expression of PTX3 in the plasma and the kidneys were assessed with urinalysis, immunohistochemical staining, and Western blot, respectively. Results. The expression of PTX3 in the kidney was significantly decreased in the DN group but increased in the IT group because of the reversal of DN. Conclusions. Our data showed that the level of PTX3 in renal tissue is closely related to renal injury in DN. This may be used to quantify the extent of renal injury in DN, provide a potential early indicator of renal tubular injury in early DN patients, and assess DN clinical progression.
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DOI：
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