In vivo induced RTX toxin ApxIVA is essential for the full virulence of Actinobacillus pleuropneumoniae
In vivo induced RTX toxin ApxIVA is essential for the full virulence of Actinobacillus pleuropneumoniae
复制标题
体内诱导的 RTX 毒素 ApxIVA 对于胸膜肺炎放线杆菌的完全毒力至关重要。
DOI：
10.1016/j.vetmic.2009.01.011
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发表时间：
2009-06-12
影响因子：
3.3
通讯作者：
Chen, Huanchun
中科院分区：
文献类型：
--
作者：
Liu, Jinlin;Chen, Xia;Chen, Huanchun
胸膜肺炎放线杆菌是一种革兰氏阴性病原体。它是猪传染性胸膜肺炎（PCP）的病原体，猪传染性胸膜肺炎是一种严重的、高度传染性的猪呼吸道疾病。在胸膜肺炎放线杆菌中已经描述了四组RTX（毒素重复序列）外毒素，其中三种已被确定为重要的毒力决定因素。本研究的目的是确定在仔猪感染胸膜肺炎放线杆菌过程中体内诱导的RTX毒素ApxIVA的致病性。基于一株缺失apxIIC的胸膜肺炎放线杆菌突变株获得了一株胸膜肺炎放线杆菌apxIVA突变株。进行了一项实验性感染试验以评估ApxIVA在仔猪中的毒力。记录了仔猪的临床症状、肺损伤评分、血液生化参数和组织病理学变化。结果表明，当存在ApxIVA时，胸膜肺炎放线杆菌的致病性更强，这表明ApxIVA对于胸膜肺炎放线杆菌完全毒力的表达是必不可少的。（C）2009由爱思唯尔出版集团出版
Actinobacillus pleuropneumoniae is a Gram-negative pathogen. It is the aetiological agent of porcine contagious pleuropneumonia (PCP), a severe and highly contagious and severe respiratory disease of swine. Four sets of RTX (repeats in toxin) exotoxins have been described in A. pleuropnuemoniae and three of them have been characterized as important virulence determinants. The aim of this study was to determine the pathogenicity of the in vivo induced RTX toxin ApxIVA during infection of piglets with A. pleuropnuemoniae. An A. pleuropnuemoniae apxIVA mutant was obtained based on an A. pleuropnuemoniae apxIIC-deleted mutant strain. An experimental infection assay was performed to evaluate the virulence of ApxIVA in piglets. Clinical signs, lung lesion scores, blood biochemical parameters and histopathologic changes in the piglets were recorded. The results indicated that the pathogenicity of A. pleuropnuemoniae was greater when ApxIVA was present, suggesting that ApxIVA is essential for expression of the full virulence of A. pleuropnuemoniae. (C) 2009 Published by Elsevier B.V.