Metabolomic profiling and biochemical evaluation of the follicular fluid of endometriosis patients
Metabolomic profiling and biochemical evaluation of the follicular fluid of endometriosis patients
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DOI：
10.1039/c7mb00181a
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发表时间：
2017-06-01
影响因子：
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通讯作者：
Emilio, Chiosi
中科院分区：
文献类型：
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作者：
Marianna, Santonastaso;Alessia, Pucciarelli;Emilio, Chiosi
疾病是复杂的系统，可以通过整合来自不同学科的数据来研究，以获得正在调查的生物现象的全局和可靠的图像。根据最近的观察，卵泡液的代谢组学分析反映了女性卵巢微环境，子宫内膜异位症是一种复杂疾病的例子，可以通过腹腔镜清楚地诊断，我们认为子宫内膜异位症患者的卵泡液可以作为一种研究模型来评估整合不同方法获得的数据的可能性。因此，这项工作的目的是通过H-1-NMR方法和体外受精（IVF）周期中不同阶段子宫内膜异位症（I-II和III-IV）女性卵泡液的生化分析，分析和整合不同的临床化学参数，具体参考代谢谱、炎症状态和细胞损伤。我们的分析表明，子宫内膜异位症患者卵泡液中磷脂、乳酸、胰岛素、PTX3、CXCL8、CXCL10、CCL11和VEGF水平高于对照组，而一些脂肪酸、赖氨酸、胆碱、葡萄糖、天门氨酸、丙氨酸、亮氨酸、缬氨酸、脯氨酸、磷脂、总LDH及其LDH-3异构体的水平低于对照组。采用RT-PCR检测患者卵母细胞周围积云细胞，检测LDHB、PTX3和胰岛素受体水平。在子宫内膜异位症患者中观察到的卵母细胞质量下降肯定与这些分子的不同水平有关。这些数据表明，不同实验方法的整合可能有助于理解复杂疾病的潜在机制，并可能导致子宫内膜异位症的更好的临床管理。
Diseases are complex systems that can be studied through the integration of data derived from different disciplines to obtain a global and reliable picture of the biological phenomenon under investigation. Based on the recent observations that the metabolomics profiling of follicular fluids reflects the ovarian microenvironment of women and that endometriosis represents an example of complex diseases, clearly diagnosed by laparoscopy, we thought that the follicular fluids of endometriosis patients can represent a study model to evaluate the possibility of integrating data obtained by different approaches. Hence, the aim of this work was to analyze and integrate different clinical chemistry parameters with specific reference to the metabolic profile, inflammatory state and cell damage by a H-1-NMR approach and biochemical analysis in the follicular fluids of women with different stages of endometriosis (I-II and III-IV) subjected to the In Vitro Fertilization (IVF) cycle. Our analysis evidenced that in the follicular fluids of endometriosis patients the levels of phospholipids, lactate, insulin, PTX3, CXCL8, CXCL10, CCL11 and VEGF were higher whereas those of some fatty acids, lysine, choline, glucose, aspartate, alanine, leucine, valine, proline, phosphocholine, total LDH as well its LDH-3 isoform were lower in comparison to the control group. The levels of LDHB, PTX3 and insulin receptor were also confirmed by RT-PCR applied on cumulus cells surrounding oocytes retrieved from the patients. The reduced oocyte quality observed in patients with endometriosis can be certainly correlated to the different levels of these molecules. These data represent how the integration of different experimental approaches may be useful for understanding the underlying mechanisms of a complex disease and can lead to a better clinical management of endometriosis.