IDENTIFICATION AND LOCALIZATION OF INTEGRIN SUBUNITS IN OOCYTES AND EGGS OF THE MOUSE
IDENTIFICATION AND LOCALIZATION OF INTEGRIN SUBUNITS IN OOCYTES AND EGGS OF THE MOUSE
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DOI：
10.1002/mrd.1080400210
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发表时间：
1995-02-01
影响因子：
2.5
通讯作者：
KOPF, GS
中科院分区：
文献类型：
--
作者：
EVANS, JP;SCHULTZ, RM;KOPF, GS
最近的研究结果表明精子中的卵整联蛋白与卵质膜结合（Blobel et al.，1991：Nature 356：248-252）。在这份报告中，免疫沉淀被用来确定，共聚焦免疫荧光显微镜被用来定位，几个不同的整合素亚基在小鼠卵细胞。α（2）、α（5）、α（v）和β（1）亚基的抗体，以及纤连蛋白受体（FNR; α（5）β（1）和/或α（3）β（1））和玻连蛋白受体（VNR; α（v）β（3）和/或α（v）β（5））的抗体，在免疫沉淀后检测适当分子量的多肽。β（1）优先定位于卵的微绒毛或无微绒毛膜/皮质区域，这些不对称定位取决于所用的抗体。由抗FNR抗体识别的蛋白质优先定位于微绒毛膜/皮质区域。使用β（1）和FNR抗体，成熟囊泡完整的卵母细胞显示出对称的质膜分布，这种不对称分布是卵母细胞成熟的结果，在中期I时可以清楚地观察到。与这些整合素亚基的膜定位相反，α（2）、α（5）和VNR主要定位于卵母细胞和卵子的细胞质中。在卵母细胞中，这些整合素亚基中的每一个均匀地分布在整个细胞质中，卵母细胞成熟与α（5）和VNR的重新分布相关，导致细胞质分布不对称，朝向纺锤体的定位增加。α（v），它位于质膜上！卵母细胞和卵子的皮质在卵母细胞成熟过程中没有显示出这种变化。这些实验的结果进行了讨论的背景下，整合素介导的精子质膜-卵子质膜的相互作用，导致卵子激活的作用。(C)1995 Wiley-Liss，Inc.
Results of a recent study have implicated egg integrins in sperm binding to the egg plasma membrane (Blobel et al., 1991: Nature 356:248-252). In this report, immunoprecipitation was used to identify, and confocal immunofluorescence microscopy was used to localize, several different integrin subunits in mouse eggs. Antibodies to alpha(2), alpha(5), alpha(v), and beta(1) subunits, as well as antibodies to the fibronectin receptor (FNR; alpha(5) beta(1) and/or alpha(3) beta(1)) and vitronectin receptor (VNR; alpha(v) beta(3) and/or alpha(v) beta(5)), detect polypeptides of the appropriate molecular weights following immunoprecipitation. beta(1) is localized preferentially to either the microvillar or amicrovillar membrane/cortical regions of eggs, and these asymmetric localizations depend on the antibody used. Proteins recognized by anti-FNR antibodies are localized preferentially to the amicrovillar membrane/cortical region. Germinal vesicle-intact oocytes display a symmetric plasma membrane distribution using beta(1) and FNR antibodies, and the asymmetric distribution develops as a consequence of oocyte maturation and is clearly observed by metaphase I. In contrast to the membrane localization of these integrin subunits, alpha(2), alpha(5), and VNR are predominantly localized in the cytoplasm of both oocytes and eggs. In the oocyte, each of these integrin subunits is uniformly distributed throughout the cytoplasm, Oocyte maturation is associated with a redistribution of alpha(5) and VNR, leading to an asymmetric cytoplasmic distribution with an increased localization towards the spindle. alpha(v), which is localized in the plasma membrane! cortex of both oocytes and eggs, does not show such a change during oocyte maturation. Results of these experiments are discussed in the context of a role for integrins in mediating sperm plasma membrane-egg plasma membrane interactions leading to egg activation. (C) 1995 Wiley-Liss, Inc.