How stable is the "Polyphyly of Lice" hypothesis (Insecta: Psocodea)?: A comparison of phylogenetic signal in multiple genes
How stable is the "Polyphyly of Lice" hypothesis (Insecta: Psocodea)?: A comparison of phylogenetic signal in multiple genes
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DOI：
10.1016/j.ympev.2010.02.026
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发表时间：
2010-06-01
影响因子：
4.1
通讯作者：
Johnson, Kevin P.
中科院分区：
文献类型：
--
作者：
Yoshizawa, Kazunori;Johnson, Kevin P.
最近的18S rDNA分子系统发育分析表明，寄生虱（Phthiraptera）可能是多系的。这些分析恢复了寄生虱亚目之一，Amblycera，作为自由生活的书虱家族Liposcelididae的姐妹群。我们进一步测试了这一假设使用的DNA序列从五个基因：核18S rDNA，组蛋白3，和无翅和线粒体16S rDNA和COL这五个基因的组合分析提供了合理的强有力的支持Amblycera + Liposcelididae分支，支持多系虱子假说。为了探索这一结果的鲁棒性，我们独立地检查了每个基因中包含的系统发育信号（除了无翅基因，它在许多目标分类群中不容易扩增）。每个基因单独和与其他基因的各种组合的分析表明，明确的信号支持Amblycera + Liposcelididae只存在于18S数据，虽然没有分析支持寄生虱的单系性。然而，所有基因的联合分析提供了更强的支持，这种关系比从18S数据单独获得。支持这个分支的增加主要是由树的其他部分的稳定性和潜在的不适当的替代建模来解释的。这些发现表明，组合数据集提供的支持值增加并不总是表明该假设得到证实。(C)2010年爱思唯尔公司All rights reserved.
Recent molecular phylogenetic analyses of 18S rDNA have indicated that parasitic lice (order Phthiraptera) are possibly polyphyletic. These analyses recovered one of the parasitic louse suborders, Amblycera, as the sister group to the free-living booklouse family Liposcelididae. We further tested this hypothesis using DNA sequences from five genes: nuclear 18S rDNA, Histone 3, and wingless and mitochondrial 16S rDNA and COL Combined analyses of these five genes provided reasonably strong support for the Amblycera + Liposcelididae clade, supporting the polyphyly of lice hypothesis. To explore the robustness of this result, we examined the phylogenetic signal contained in each gene independently (except for wingless, which could not be readily amplified in many target taxa). Analyses of each gene separately and in various combinations with other genes revealed that clear signal supporting Amblycera + Liposcelididae only existed in the 18S data, although no analysis supported monophyly of parasitic lice. Nevertheless, combined analyses of all genes provided stronger support for this relationship than that obtained from 18S data alone. The increase in support for this clade was mostly explained by the stabilization of other parts of the tree and potentially inappropriate substitution modeling. These findings demonstrate that the increased support values provided by combined data set does not always indicate corroboration of the hypothesis. (C) 2010 Elsevier Inc. All rights reserved.