Characterization of gastric mucosal membranes: lipid composition of purified gastric microsomes from pig, rabbit, and frog.
Characterization of gastric mucosal membranes: lipid composition of purified gastric microsomes from pig, rabbit, and frog.
复制标题
胃粘膜的表征：猪、兔和青蛙纯化胃微粒体的脂质成分。
DOI：
10.1016/0003-9861(79)90530-7
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发表时间：
1979
影响因子：
3.9
通讯作者：
T. Ray
中科院分区：
文献类型：
--
作者：
P. Sen;T. Ray
测定了猪、兔和青蛙胃底粘膜梯度纯化的胃微粒体膜的脂质成分。总脂质含量差异很大。与兔子相比（21.6 ± 0.6 毫克/100 毫克蛋白质），猪的脂质含量约为兔子的两倍，青蛙的脂质含量约为兔子的三倍。两种哺乳动物的胆固醇水平（约占脂质的 32%）均高于青蛙（23%）。磷脂约占猪、兔和蛙微粒体总脂质的45%、54%和52%，这三个物种中胆固醇与磷脂的摩尔比分别为1.95、1.6和1.17。磷脂酰胆碱和磷脂酰乙醇胺合计约占猪和青蛙总磷脂的75%，以及兔胃微粒体中的93%。猪、兔和青蛙中的鞘磷脂分别占 19.3%、3.2% 和 1.5%。猪、兔和青蛙中磷脂酰肌醇分别占5%、2.7%和23.6%。猪、兔和青蛙体内磷脂酰乙醇胺与磷脂酰胆碱的比例分别为1.17、1.1和0.85。饱和脂肪酸16:0和18:0以及不饱和脂肪酸18:1和18:2是所有磷脂中的主要脂肪酸。所有三个物种的磷脂酰胆碱中饱和脂肪酸与不饱和脂肪酸的比率在0.8和0.9之间，磷脂酰乙醇胺中的饱和脂肪酸与不饱和脂肪酸的比率在0.27和0.5之间。所有物种的磷脂酰肌醇和鞘磷脂中饱和脂肪酸的贡献远大于磷脂酰胆碱和磷脂酰乙醇胺。磷脂酰胆碱的1位有63%饱和脂肪酸和37%不饱和脂肪酸；而位置 2 则相反。然而，磷脂酰乙醇胺的位置 1 含有 85% 的饱和脂肪酸，而位置 2 的饱和脂肪酸仅为 25%。花生四烯酸 (20:4) 在仅位于磷脂酰胆碱和磷脂酰乙醇胺的位置 2 的所有物种中都大量存在。
The lipid compositions of the gradient-purified gastric microsomal membranes from the fundic mucosa of pig, rabbit, and frog were determined. The total lipid content varied widely. Compared to the rabbit (21.6 ± 0.6 mg/100 mg protein), the pig had about twice as much and the frog about three times as much lipid. The levels of cholesterol were higher in both mammalian species (about 32% of the lipid) compared to frog (23%). Phospholipids accounted for about 45, 54, and 52% of the total microsomal lipids from pig, rabbit, and frog and the molar ratios of cholesterol to phospholipid in the three species were 1.95, 1.6, and 1.17, respectively. Phosphatidyl choline and phosphatidyl ethanolamine together constituted about 75% of the total phospholipids in pig and frog and 93% in rabbit gastric microsomes. Sphingomyelin comprised 19.3, 3.2, and 1.5% in pig, rabbit, and frog, respectively. Phosphatidyl inositol constituted 5, 2.7, and 23.6% in pig, rabbit, and frog, respectively. The ratios of phosphatidyl ethanolamine to phosphatidyl choline were 1.17, 1.1, and 0.85 in pig, rabbit, and frog, respectively. The saturated fatty acids 16:0 and 18:0 and the unsaturated fatty acid 18:1 and 18:2 were the predominant fatty acids in all phospholipids. The ratios of saturated to unsaturated fatty acids were between 0.8 and 0.9 in phosphatidyl choline and 0.27 and 0.5 in phosphatidyl ethanolamine from all three species. The contributions by saturated fatty acids were much more in phosphatidyl inositol and sphingomyelin than in phosphatidyl choline and phosphatidyl ethanolamine from all species. Position 1 of phosphatidyl choline had 63% saturated and 37% unsaturated fatty acids; while the reverse was true for position 2. Phosphatidyl ethanolamine, however, had 85% saturated fatty acids in position 1 compared to only 25% in position 2. Arachidonic acid (20:4) was present in significant amounts in all species located exclusively at position 2 of both phosphatidyl choline and phosphatidyl ethanolamine.