Reduced production of diacetyl by overexpressing BDH2 gene and ILV5 gene in yeast of the lager brewers with one ILV2 allelic gene deleted
Reduced production of diacetyl by overexpressing BDH2 gene and ILV5 gene in yeast of the lager brewers with one ILV2 allelic gene deleted
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通过在删除了一个 ILV2 等位基因的啤酒啤酒酵母中过度表达 BDH2 基因和 ILV5 基因，减少了二乙酰的产生
DOI：
10.1007/s10295-017-1903-6
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发表时间：
2017-03-01
影响因子：
3.4
通讯作者：
Xiao, Dong-Guang
中科院分区：
文献类型：
--
作者：
Shi, Ting-Ting;Li, Ping;Xiao, Dong-Guang
双乙酰在啤酒中会产生不想要的黄油异味。在啤酒发酵过程中，通过改变酵母的代谢途径来减少双乙酰的产生。本研究利用PGK1启动子在酿酒酵母中表达了BDH2和ILV5基因，它们分别编码二乙酰还原酶和乙酰羟基酸还原异构酶，缺失了一个ILV2等位基因。对双乙酰含量和发酵性能进行了检测和比较。结果表明，QI2-KP(缺失1个ILV2等位基因)、QI2-B2Y(高表达BDH2基因、缺失1个ILV2等位基因)和QI2-I5Y(高表达ILV5基因、缺失1个ILV2等位基因)啤酒中双乙酰含量分别显著降低16.52%、55.65%和69.13%。突变株的发酵能力与S2相似。本研究结果可为该技术的进一步发展及其在科学研究和工业啤酒生产中的广泛应用提供参考。
Diacetyl causes an unwanted buttery off-flavor in lager beer. The production of diacetyl is reduced by modifying the metabolic pathway of yeast in the beer fermentation process. In this study, BDH2 and ILV5 genes, coding diacetyl reductase and acetohydroxy acid reductoisomerase, respectively, were expressed using a PGK1 promoter in Saccharomyces cerevisiae, which deleted one ILV2 allelic gene. Diacetyl contents and fermentation performances were examined and compared. Results showed that the diacetyl content in beer was remarkably reduced by 16.52% in QI2-KP (one ILV2 allelic gene deleted), 55.65% in QI2-B2Y (overexpressed BDH2 gene and one ILV2 allelic gene deleted), and 69.13% in QI2-I5Y (overexpressed ILV5 gene and one ILV2 allelic gene deleted) compared with the host strain S2. The fermentation ability of mutant strains was similar to that of S2. Results of the present study can lead to further advances in this technology and its broad application in scientific investigations and industrial beer production.