Oxidative stress causes reversible changes in mitochondrial permeability and structure.
Oxidative stress causes reversible changes in mitochondrial permeability and structure.
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DOI：
10.1016/j.exger.2010.01.016
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发表时间：
2010-08
影响因子：
3.9
通讯作者：
Kim, Geumsoo
中科院分区：
文献类型：
--
作者：
Cole, Nelson B.;Daniels, Mathew P.;Levine, Rodney L.;Kim, Geumsoo
关键词：
线粒体是细胞内活性氧的主要来源和主要目标。使用免疫荧光显微镜，我们发现在用胆固醇结合洗涤剂皂苷透化的甲醛固定细胞中通常检测不到许多线粒体基质蛋白。然而，固定前施加的外源或内源氧化应激改变了线粒体的通透性，使这些基质蛋白易于被抗体接触。电子显微镜显示氧化应激导致基质密度损失和内膜嵴混乱。值得注意的是，当氧化应激缓解时，渗透性和结构的变化迅速逆转。活性氧可逆地改变线粒体膜的渗透性的能力为细胞内（例如细胞核和线粒体之间）的通讯提供了潜在的机制。
Mitochondria are a primary source as well a principal target of reactive oxygen species within cells. Using immunofluorescence microscopy, we have found that a number of mitochondrial matrix proteins are normally undetectable in formaldehyde fixed cells permeabilized with the cholesterol-binding detergent saponin. However, exogenous or endogenous oxidative stress applied prior to fixation altered the permeability of mitochondria, rendering these matrix proteins accessible to antibodies. Electron microscopy revealed a loss of matrix density and disorganization of inner-membrane cristae upon oxidative stress. Notably, the changes in permeability and in structure were rapidly reversed when the oxidative stress was relieved. The ability of reactive oxygen species to reversibly alter the permeability of the mitochondrial membrane provides a potential mechanism for communication within the cell such as between nucleus and mitochondria.
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影响因子：
4.8
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