Colloidal Au replacement assay for highly sensitive quantification of low molecular weight analytes by surface plasmon resonance.
Colloidal Au replacement assay for highly sensitive quantification of low molecular weight analytes by surface plasmon resonance.
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DOI：
10.1021/bc0603541
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发表时间：
2007-06
影响因子：
4.7
通讯作者：
S. Takae;Y. Akiyama;Y. Yamasaki;Y. Nagasaki;K. Kataoka
中科院分区：
文献类型：
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作者：
S. Takae;Y. Akiyama;Y. Yamasaki;Y. Nagasaki;K. Kataoka
开发了一种基于表面等离子共振 (SPR) 的新型传感方法，用于低分子量 (LMW) 分析物的高灵敏度定量（胶体金替代测定）。制备了用乳糖基聚乙二醇 (PEG) 功能化的金纳米颗粒（直径 = 20 nm），并将其特异性吸附到固定化的蓖麻凝集素 (RCA120) 的 SPR 传感器芯片表面上。随后注射游离的 d-半乳糖，以与半乳糖浓度成比例的方式引发预吸附的乳糖基聚乙二醇化金纳米颗粒的洗脱，从而在较宽的半乳糖浓度范围（0.1-50 ppm）内实现实质性定量分析。这种通过从传感器芯片表面替代洗脱预吸附纳米颗粒进行 d-半乳糖传感的方法将适用于各种 LMW 分析物的高灵敏度 SPR 定量，已知这些分析物很难通过传统的 SPR 传感方式检测。
A novel sensing method based on surface plasmon resonance (SPR) was developed for the highly sensitive quantification of low molecular weight (LMW) analytes (colloidal Au replacement assay). Gold nanoparticles (diameter = 20 nm) functionalized with lactosyl-poly(ethylene glycol) (PEG) were prepared and were specifically adsorbed onto a Ricinus communis agglutinin (RCA120)-immobilized SPR sensor chip surface. Subsequent injection of free d-galactose elicited the elution of the preadsorbed lactosyl-PEGylated gold nanoparticles in a manner proportional to the galactose concentration, achieving a substantial and quantitative analysis over a wide range of galactose concentrations (0.1-50 ppm). This method of d-galactose sensing through the substituted elution of preadsorbed nanoparticles from the sensor chip surface would be applicable for the highly sensitive SPR quantification of various LMW analytes, which are known to be difficult to detect by the conventional SPR sensing regime.