Anti-inflammatory, analgesic and anti-tumor properties of gold nanoparticles
Anti-inflammatory, analgesic and anti-tumor properties of gold nanoparticles
复制标题
DOI：
10.1016/j.pharep.2016.09.017
复制
发表时间：
2017-01-01
影响因子：
4.4
通讯作者：
Gasparotto, Luiz H. S.
中科院分区：
文献类型：
--
作者：
de Araujo Junior, Raimundo Fernandes;de Araujo, Aurigena Antunes;Gasparotto, Luiz H. S.
背景：金纳米颗粒（GNP）被认为是潜在的药物载体。然而，在将它们用作药物载体之前，必须了解它们与活生物体的相互作用。本研究报道了GNP的抗炎、镇痛和抗肿瘤作用。方法：采用环境友好的方法合成GNP，并用TEM和UV-vis对GNP进行表征。GNP作用于HT-29细胞后，Annexin V和碘化丙啶染色检测细胞凋亡，激光共聚焦显微镜检测caspase-3活性。将GNP施用给雄性和雌性瑞士小鼠，用于对其镇痛和镇痛性质的后验评估。结果：GNP浓度在40 μ g/mL ~ 80 μ g/mL范围内，细胞凋亡呈剂量依赖性（p < 0.05）。在1500 μ g/kg剂量下观察到最佳抗炎活性，其导致白细胞迁移减少49.3%。GNP在1500 μ g/kg的剂量下显示外周镇痛，并且已经在肝、脾、肾和肺中发现。组织学检查发现肺中的红细胞外渗。结论：这项研究提请注意金纳米粒子作为一种资源，在抗炎，镇痛和抗肿瘤领域的技术创新。GNPs具有生物效应，值得调查，以评估其与有机系统的充分相互作用。(C)2016由Elsevier Sp. z o.o.出版代表波兰科学院药理学研究所。
Background: Gold nanoparticles (GNPs) are regarded as potential platforms for drug delivery. However, their interaction with live organisms must be understood prior to their utilization as drug carriers. The present study reports the anti-inflammatory, analgesic and anti-tumor effects of GNPs. The biodistribution of GNPs and their effect on various tissues have also been studied.Methods: GNPs were synthesized through an environmentally friendly route and characterized with TEM and UV-vis. After HT-29 cells had been exposed to GNPs, apoptosis was assessed with Annexin V and propidium iodide staining and caspase-3 activity determined with a confocal laser scanning microscope. GNPs were administrated to male and female Swiss mice for posterior assessment of their antiinflammatory and analgesic properties. The biodistribution of GNPs and their impact on tissues were studied with UV-vis and histopathological analysis, respectively.Results: Cell apoptosis was observed in a dose-dependent manner for GNPs concentrations ranging from 40 mu g/mL to 80 mu g/mL (p < 0.05). The best anti-inflammatory activity was observed at the dose of 1500 mu g/kg, which caused a reduction of 49.3% in leukocyte migration. GNPs showed peripheral analgesia at the dose of 1500 mu g/kg and have been found in liver, spleen, kidney and lungs. Histopathological examination revealed extravasation of red blood cells in lungs.Conclusion: The study draws attention to gold nanoparticles as a resource for technological innovation in the anti-inflammatory, analgesic and anti-tumor fields. GNPs have biological effects that deserve investigation to assess their full interaction with organic systems. (C) 2016 Published by Elsevier Sp. z o.o. on behalf of Institute of Pharmacology, Polish Academy of Sciences.