Thymocyte subsets transformed by Abelson murine leukemia virus
Thymocyte subsets transformed by Abelson murine leukemia virus
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阿贝尔森鼠白血病病毒转化的胸腺细胞亚群
DOI：
10.1128/mcb.5.2.390-397.1985
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发表时间：
1985
影响因子：
5.3
通讯作者：
W. Cook
中科院分区：
文献类型：
--
作者：
W. Cook
用放射性白血病病毒（RadLV）取代其通常的辅助病毒Moloney白血病病毒，改变了Abelson小鼠白血病病毒的感染复合体。胸腺内注射Abelson-RadLV复合体后，胸腺瘤迅速出现，如先前所述，注射Abelson-Moloney复合体。从每个Abelson病毒复合体诱导的胸腺瘤中获得细胞系，并根据正常胸腺细胞表型进行分类。每种病毒复合体诱导的细胞系不能表达Thy-1细胞表面抗原，相当于小鼠胸腺细胞的0.7%亚群。到目前为止，这些细胞系与分类为前b细胞的一些abelson衍生的骨髓转化细胞无法区分。然而，Abelson-Moloney复合体诱导的细胞系表达Thy-1水平较低，与胸腺髓质未成熟母细胞具有大部分相同的标记物，而Abelson-RadLV复合体诱导的细胞系则与胸腺皮质母细胞明显相似。因此，Abelson病毒可以诱导至少两种，也可能是三种不同表型的胸腺瘤细胞系，与正常胸腺细胞母细胞亚群相对应，其中哪一种表型的确定可能受到辅助病毒序列的影响。
The infectious complex of Abelson murine leukemia virus was altered by replacing its usual helper virus, Moloney leukemia virus, with radiation leukemia virus (RadLV). After intrathymic injection of the Abelson-RadLV complex, thymomas arose rapidly, as described previously for injection of the Abelson-Moloney complex. Cell lines were derived from thymomas induced by each Abelson virus complex and were classified according to normal thymus cell phenotypes. Each virus complex induced some cell lines which were like a 0.7% subpopulation of murine thymocytes in that they failed to express the Thy-1 cell-surface antigen. These lines are thus far indistinguishable from some Abelson-derived bone marrow transformants classified as pre-B cells. However, the Abelson-Moloney complex induced some cell lines which expressed low levels of Thy-1 and which shared most markers with immature blast cells of the thymic medulla, whereas the Abelson-RadLV complex induced some lines which were clearly like thymic cortex blast cells. Thus, Abelson virus can induce thymoma cell lines of at least two, and possibly three, distinct phenotypes corresponding to normal thymocyte blast subsets, the determination of which can be influenced by helper virus sequences.
胸腺细胞亚群：实验综述，包括主要小鼠胸腺细胞标记物之间的流式细胞术互相关性。
DOI：
--
发表时间：
1983
期刊：
Thymus
影响因子：
--
作者：
Scollay,R;Shortman,K
通讯作者：
Shortman,K