Spatial and temporal distribution of Oct-4 and acetylated H4K5 in rabbit embryos.
Spatial and temporal distribution of Oct-4 and acetylated H4K5 in rabbit embryos.
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DOI：
10.1016/j.rbmo.2012.01.001
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发表时间：
2012-04
影响因子：
4
通讯作者：
Xu J
中科院分区：
文献类型：
--
作者：
Chen CH;Chang WF;Liu CC;Su HY;Shyue SK;Cheng WT;Chen YE;Wu SC;Du F;Sung LY;Xu J
兔是研究人类胚胎学的一个独特物种，但关于兔胚胎关键转录因子和表观遗传事件的研究报道有限。本研究应用免疫组化染色技术检测了兔胚胎中Oct-4和乙酰化H4 K5（H4 K5 ac）的表达模式。受精后，整个胚胎细胞核中Oct-4信号的平均强度激增，然后下降，直到8细胞期，然后增加，直到致密桑葚胚（CM）期。此后，从CM阶段到早期囊胚（EB）阶段，它降低，在扩张囊胚（EXPB）阶段达到最低值，并回到与孵化囊胚（HB）中的CM阶段胚胎相似的水平。Oct-4信号在囊胚的内细胞团（ICM）和滋养外胚层（TE）细胞中都有表达。整个胚胎的平均H4 K5 ac信号强度在受精后增加，在4-细胞期开始下降，在8-细胞期达到最小值，在EXPB期再次增加，在HB期达到峰值。虽然TE细胞在整个胚泡阶段保持相似的H4 K5 ac水平，但HB的ICM细胞显示出比EB和EXPB更高的H4 K5 ac水平。
Rabbit is a unique species to study human embryology; however, there are limited reports on the key transcription factors and epigenetic events of rabbit embryos. This study examined the Oct-4 and acetylated H4K5 (H4K5ac) patterns in rabbit embryos using immunochemistry staining. The average intensity of the Oct-4 signal in the nuclei of the whole embryo spiked upon fertilization, then decreased until the 8-cell stage and increased afterwards until the compact morula (CM) stage. It decreased thereafter from the CM stage to the early blastocyst (EB) stage, with a minimum at the expanded blastocyst (EXPB) stage and came back to a level similar to that of the CM-stage embryos in the hatching blastocysts (HB). The Oct-4 signal was observed in both the inner cell mass (ICM) and the trophectoderm (TE) cells of blastocysts. The average H4K5ac signal intensity of the whole embryo increased upon fertilization, started to decrease at the 4-cell stage, reached a minimum at the 8-cell stage, increased again at the EXPB stage and peaked at the HB stage. While TE cells maintained similar levels of H4K5ac throughout the blastocyst stages, ICM cells of HB showed higher levels of H4K5ac than those of EB and EXPB.
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影响因子：
--
作者：
Kobolak J;Kiss K;Polgar Z;Mamo S;Rogel-Gaillard C;Tancos Z;Bock I;Baji AG;Tar K;Pirity MK;Dinnyes A
通讯作者：
Dinnyes A
影响因子：
4
作者：
Cauffman, G;Van de Velde, H;Van Steirteghem, A
通讯作者：
Van Steirteghem, A
影响因子：
3.6
作者：
Kurosaka, S;Eckardt, S;McLaughlin, KJ
通讯作者：
McLaughlin, KJ
影响因子：
3.6
作者：
Mitalipov, SM;Kuo, HC;Wolf, DP
通讯作者：
Wolf, DP
影响因子：
5.3
作者：
Chew, JL;Loh, YH;Ng, HH
通讯作者：
Ng, HH