A comparison of several lines of transgenic mice containing the SV40 early genes.
A comparison of several lines of transgenic mice containing the SV40 early genes.
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几个含有 SV40 早期基因的转基因小鼠品系的比较。
DOI：
10.1101/sqb.1985.050.01.082
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Levine,AJ
中科院分区：
文献类型：
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作者：
VanDyke,T;Finlay,C;Levine,AJ
将猿猴病毒40 （SV40）接种到新生仓鼠体内可导致来自多种组织类型的广谱肿瘤（Tooze 1981）。皮下注射病毒通常会导致纤维肉瘤（Eddy et al. 1961）；颅内接种可产生神经胶质瘤、脉络膜丛乳头状瘤和室管膜瘤（Gerber and Kirschstein 1962）；静脉注射引起白血病、淋巴瘤、骨肉瘤和网状细胞肉瘤（Diamandopoulos 1972,1978）。在小鼠中，用SV40诱导肿瘤的尝试通常失败（见Tooze 1981）。然而，最近的实验表明，在病毒持续存在和宿主免疫反应改变的特殊条件下，可能会出现小肿瘤（Ambramczuk et al. 1984）。另一方面，当SV40早期区基因被引入小鼠种系时，65-90~ 70的转基因小鼠发生了特定组织类型的肿瘤，脉膜丛(Brinster et al. 1984和pers。通讯)。在这些转基因小鼠中，SV40大T抗原的表达通常局限于脉络膜丛肿瘤。部分小鼠肾和胸腺表达低水平T抗原，偶见肾皮质囊肿和胸腺增生（Brinster et al. 1984）。携带SV40缺失的转基因小鼠。早期区域研究表明，脉络膜丛乳头状瘤的产生需要大T抗原，而不是小T抗原（Palmiter et al. 1985；表1）。SV40增强子区，即72 bp重复序列（Gruss et al. 1981）在脉络膜丛乳头状瘤的组织特异性诱导中发挥作用，但在肿瘤发生中不是必需的（Palmiter et al. t985）。与T抗原基因的杂交基因结构已被用来证明5'-侧翼序列在控制组织中的一般作用
Inoculation of simian virus 40 (SV40) into newborn hamsters can result in a broad spectrum of tumors derived from many tissue types (Tooze 1981). Subcutaneous injections of virus usually result in fibrosarcomas (Eddy et al. 1961); intracranial inoculations may produce gliomas, papillomas of the choroid plexus, and ependynomas (Gerber and Kirschstein 1962); and intravenous injections have given rise to leukemias, lymphomas, osteosarcomas, and reticulum cell sarcomas (Diamandopoulos 1972, 1978). In mice, attempts to induce tumors with SV40 have usually failed (see Tooze 1981). However, recent experiments suggest that small tumors may arise under special conditions of viral persistence and an altered host immune response (Ambramczuk et al. 1984). On the other hand, when the SV40 early-region genes were introduced into the germ line of mice, 65-90~ 70 of the transgenic mice developed tumors of a specific tissue type, the choroid plexus (Brinster et al. 1984 and pers. comm.). In these transgenie mice the expression of the SV40 large T antigen was generally limited to the choroid plexus tumors. Some mice expressed a low level of T antigen in the kidney and thymus, and cortical cysts of the kidney and thymic hyperplasia were observed occasionally (Brinster et al. 1984).Transgenic mice carrying deletions in the SV40. early region have served to demonstrate that the large T antigen, but not the small t antigen, is required for the production of choroid plexus papillomas (Palmiter et al. 1985; Table 1). It also appears that the SV40 enhancer region, the 72-bp repeat (Gruss et al. 1981), plays a role in the tissue-specific induction of choroid plexus papillomas but it is not essential for tumorigenesis (Palmiter et al. t985). Hybrid gene constructs with the T antigen gene have been used to demonstrate the general role of 5'-flanking sequences in controlling tissue