Effect of Allium sativum and Allium cepa oils on different stages of Boophilus annulatus
Effect of Allium sativum and Allium cepa oils on different stages of Boophilus annulatus
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DOI：
10.1007/s00436-013-3344-0
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发表时间：
2013-05-01
影响因子：
2
通讯作者：
Shokier, K. A.
中科院分区：
文献类型：
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作者：
Aboelhadid, S. M.;Kamel, A. A.;Shokier, K. A.
研究了大蒜油和洋葱油对不同虫态的环状牛蜱的杀螨效果。充血的B。从自然感染的牛中收集annulatus雌性。在28 A ° C和85%相对湿度下孵育许多饱食的蜱以产卵，孵育这些卵以获得用于研究的幼虫。采用水蒸气蒸馏法提取大蒜油和洋葱油，用气相色谱法对大蒜油和洋葱油进行分析。将这些油溶解在乙醇、甲醇和部分水中。以不同浓度测试油; 1、2、5、10和20%。通过成虫浸泡试验将这些浓度应用于成虫蜱;通过幼虫浸泡技术将这些浓度应用于幼虫蜱和卵。20%、10%和5%的大蒜油乙醇和甲醇稀释液在24小时内杀死所有蜱虫成虫和幼虫。对于溶解在水中的10%和20%大蒜油获得了类似的结果。10%大蒜油水溶液对含胚卵的影响是明确的，因为其添加到这些卵中导致其孵化能力降低、形状变形和颜色变化。乙醇和甲醇中的10和20%洋葱油在施用后72小时内杀死76- 86%的成蜱。两种浓度处理后24 h内幼虫全部死亡。这些浓度（10%和20%）的洋葱油在水中杀死了56- 80%的治疗蜱。此外，10%的洋葱油水溶液阻止了胚胎卵的孵化。我们得出结论，大蒜和洋葱油对B的所有阶段都有杀螨作用。浓度高于5%的环纹藻。只有大蒜油在5%的酒精浓度下才能杀死100%的成年蜱。
The study is aimed to investigate the acaricidal effect of Allium sativum (garlic) and Allium cepa (onion) oils on different stages of Boophilus annulatus hard tick. Engorged B. annulatus females were collected from naturally infected cattle. A number of engorged ticks were incubated at 28 A degrees C and 85 % relative humidity to lay eggs, which were incubated to obtain larvae that were used in the study. The used garlic and onion oils were prepared by steam distillation and were analyzed by gas chromatography. These oils were dissolved in ethanol, methanol alcohols, and, partially, in water. The oils were tested in different concentrations; 1, 2, 5, 10, and 20 %. These concentrations were applied on adult ticks by adult immersion test; on larvae by larval immersion technique and on eggs. The 20, 10, and 5 % of garlic oil dilutions in ethanol and methanol killed all adult ticks and larvae within 24 h. Similar results were obtained for 10 and 20 % garlic oil dissolved in water. The effect of 10 % aqueous solution of garlic oil on embryonated eggs was clear as its addition to these eggs led to their in ability to hatch, deformity in shape, and change in color. The 10 and 20 % onion oil in ethanol and methanol alcohols killed 76-86 % of the adult ticks within 72 h post-application. While, all larvae died within 24 h postsubjected to these two concentrations. These concentrations (10 and 20 %) of onion oil in water killed 56-80 % of the treated ticks. Moreover, 10 % aqueous solution of onion oil prevented hatching of embyonated eggs. We concluded that garlic and onion oils have acaricidal effect on all stages of B. annulatus at concentrations higher than 5 %. Only garlic oil could kill 100 % of adult ticks at concentrations from 5 % in alcohols.