Translation of Diverse Aramid- and 1,3-Dicarbonyl-peptides by Wild Type Ribosomes in Vitro
Translation of Diverse Aramid- and 1,3-Dicarbonyl-peptides by Wild Type Ribosomes in Vitro
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DOI：
10.1021/acscentsci.9b00460
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发表时间：
2019-07-24
影响因子：
18.2
通讯作者：
Schepartz, Alanna
中科院分区：
文献类型：
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作者：
Ad, Omer;Hoffman, Kyle S.;Schepartz, Alanna
在这里，我们报告说，野生型大肠杆菌核糖体接受并延长用多种苯甲酸（包括芳族聚酰胺前体）以及丙二酸基（1，3-二羰基）底物酰化的预充电引发剂tRNA，以产生一系列不同的芳族聚酰胺肽和聚酮肽-肽杂合分子。这项工作扩大了核酶和核糖体催化的化学转化的范围，提供了一个起点，在体内翻译工程的努力，并提供了一种替代策略的聚酮肽天然产物的生物合成。
Here, we report that wild type Escherichia coli ribosomes accept and elongate precharged initiator tRNAs acylated with multiple benzoic acids, including aramid precursors, as well as malonyl (1,3-dicarbonyl) substrates to generate a diverse set of aramid-peptide and polyketide-peptide hybrid molecules. This work expands the scope of ribozyme-and ribosome-catalyzed chemical transformations, provides a starting point for in vivo translation engineering efforts, and offers an alternative strategy for the biosynthesis of polyketide-peptide natural products.