Long-term preservation of antigenicity on tissue microarrays
Long-term preservation of antigenicity on tissue microarrays
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DOI：
10.1038/labinvest.3700131
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发表时间：
2004-08-01
影响因子：
5
通讯作者：
Camp, RL
中科院分区：
文献类型：
--
作者：
DiVito, KA;Charette, LA;Camp, RL
组织微阵列促进了大型队列研究的评估；然而，关于保存切片的最佳方法的数据很少。我们评估了三种储存预切乳腺癌微阵列载玻片的方法：石蜡涂层和氮干燥器储存，单独或联合使用。我们测试了三种抗原，细胞角蛋白，雌激素受体和Ki-67在室温下储存3个月的微阵列上的耐久性。采用传统棕色染色法（0-3+）进行人工评分，以及采用荧光染色切片进行自动评分。将染色强度与新切片的染色强度进行比较。在环境条件下（室温和空气）储存3个月的载玻片显示出所有靶抗原的明显降解，在某些情况下导致载玻片几乎不可读。我们发现石蜡包覆和氮储存相结合可以最好地保存抗原性，根据所使用的标记和检测系统的不同，新切割的载玻片的抗原性保留率为72-99%。单独使用石蜡涂层或氮气储存对载玻片的保护程度较低。
Tissue microarrays have facilitated the evaluation of large cohort studies; however, there is little data on the best method for preserving sections once they are cut. We assessed three methods of storing precut breast cancer microarray slides: paraffin coating and storage in a nitrogen desiccator, either alone or in combination. We tested the durability of three antigens, cytokeratin, estrogen receptor, and Ki-67 on microarrays stored under these conditions for 3 months at room temperature. Staining was assessed with both manual scoring using traditional brown stain (0-3+) as well as automated scoring using fluorescently stained sections. Staining intensity was compared to that obtained from freshly cut slides. Slides stored under ambient conditions (room temperature and air) for 3 months exhibited marked degradation of all target antigens, in some cases resulting in slides that were virtually unreadable. We found that combined paraffin coating and nitrogen storage resulted in the best preservation of antigenicity, with retention of 72-99% of the antigenicity of a freshly cut slide, depending upon the marker and detection system used. The use of either paraffin coating or nitrogen storage alone protected slides to a lesser degree.