Confirmation and characterization of cyhalofop-butyl-resistant Chinese sprangletop (Leptochloa chinensis) populations from China
Confirmation and characterization of cyhalofop-butyl-resistant Chinese sprangletop (Leptochloa chinensis) populations from China
复制标题
中国抗氰氟草酯的中国千金草种群的确认和鉴定
DOI：
10.1017/wsc.2020.15
复制
发表时间：
2020-05-01
期刊：
影响因子：
2.5
通讯作者：
Bai Lianyang
中科院分区：
文献类型：
--
作者：
Peng Yajun;Pan Lang;Bai Lianyang
中国雀儿头[Leptoplata chinensis（L.）Nees]是一种严重危害水稻的禾本科杂草。在中国的水稻种植。氰氟草酯是我国水稻田主要防除禾本科杂草的除草剂，尤其是对禾本科杂草的防除效果最好。chinensis，L.由于持续和广泛的应用，中国已进化出对氰氟草酯的抗性。研究了苜蓿对氰氟草酯的抗性水平及其抗性机制。在中国部分稻区的中国稻瘟病菌中，收集了66个田间种群，并用氰氟草酯处理。在这些受试种群中，有10个种群对氰氟草酯表现出高水平的抗性;与易感种群相比，50%有效剂量范围为108.4至1，443.5 g ai ha（-1），抗性指数值为9.1至121.8。对感病群体和全部10个抗性群体的乙酰辅酶A羧化酶基因（ACCase）进行了扩增和测序。其中，在5个耐药群体中发现Ile-1781-Leu、Trp-2027-Cys、Trp-2027-Ser和Ile-2041-Asn突变。在其他5个抗性群体中未发现已知的抗性相关突变，表明这些群体对氰氟草酯的抗性可能是由非靶位点抗性机制赋予的。值得注意的是，Ile-1781-Leu和Trp-2027-Cys替换先前已被报道，但这是L中Trp-2027-Ser和Ile-2041-Asn突变的首次报道。chinensis。此外，还建立了三种衍生的切割扩增多态性序列方法，用于快速检测L。中国。
Chinese sprangletop [Leptochloa chinensis (L.) Nees] is one grass weed severely affecting rice (Oryza sativa L.) growth in paddies in China. Cyhalofop-butyl is the main herbicide used to control grass weeds in Chinese paddy fields, especially for controlling L. chinensis; however, L. chinensis has evolved resistance to cyhalofop-butyl due to continuous and extensive application. To investigate cyhalofop-butyl resistance levels and mechanisms in L. chinensis in some of the Chinese rice areas, 66 field populations were collected and treated with cyhalofop-butyl. Of these tested populations, 10 showed a high level of resistance to cyhalofop-butyl; the 50% effective dose ranged within 108.4 to 1,443.5 g ai ha(-1) with resistance index values of 9.1 to 121.8 when compared with the susceptible population. Acetyl-coenzyme A carboxylase genes (ACCase) of susceptible and all 10 resistant populations were amplified and sequenced. Among them, Ile-1781-Leu, Trp-2027-Cys, Trp-2027-Ser, and Ile-2041-Asn mutations were found in five resistant populations. No known resistance-related mutations were found in the other five resistant populations, indicating that resistance to cyhalofop-butyl in these populations was likely to be endowed by non-target site resistance mechanisms. Notably, the Ile-1781-Leu and Trp-2027-Cys substitutions have previously been reported, but this is the first report of Trp-2027-Ser and Ile-2041-Asn mutations in L. chinensis. Furthermore, three derived cleaved amplified polymorphic sequence methods were developed to rapidly detect these mutations in L. chinensis.