Flow Cytometric Analysis of Lymphocyte Proliferative Responses to Food Allergens in Dogs with Food Allergy
Flow Cytometric Analysis of Lymphocyte Proliferative Responses to Food Allergens in Dogs with Food Allergy
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DOI：
10.1292/jvms.10-0410
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发表时间：
2011-10-01
影响因子：
1.2
通讯作者：
Okayama, Taro
中科院分区：
文献类型：
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作者：
Fujimura, Masato;Masuda, Kenichi;Okayama, Taro
采用抗原特异性免疫球蛋白E定量（IgE试验）和外周血淋巴细胞抗原特异性增殖的流式细胞术分析（淋巴细胞增殖试验）两种不同的过敏试验，研究食物过敏犬（FA）对食物过敏原的过敏反应差异。根据临床结果和消除饮食试验，13只狗被诊断为FA。选取临床诊断为犬特应性皮炎（CAD）的犬7只作为疾病对照组，健康犬5只作为阴性对照组。FA组分别用血清IgE试验和淋巴细胞增殖试验鉴定出19例和33例过敏原反应。同样，在CAD组中，每次测试分别发现12和6个过敏原反应，在健康犬中，每次测试分别发现3和0个过敏原反应。淋巴细胞增殖试验发现FA与健康犬在过敏原检测阳性方面存在显著差异，表明该试验可用于区分FA与健康犬，但不能用于区分CAD。在经过1.5至5个月的消除饮食试验后，6只患有FA的狗重复了这两项测试。11例阳性反应中有9例的IgE浓度下降了20 ~ 80%，而淋巴细胞增殖试验的阳性反应全部下降到接近于零(P
Two different allergy tests, antigen-specific immunoglobulin E quantification (IgE test) and flow cytometric analysis of antigen-specific proliferation of peripheral lymphocytes (lymphocyte proliferation test), were performed to examine differences in allergic reactions to food allergens in dogs with food allergy (FA). Thirteen dogs were diagnosed as FA based on clinical findings and elimination diet trials. Seven dogs clinically diagnosed with canine atopic dermatitis (CAD) were used as a disease control group, and 5 healthy dogs were used as a negative control group. In the FA group, 19 and 33 allergen reactions were identified using the serum IgE test and the lymphocyte proliferation test, respectively. Likewise, in the CAD group, 12 and 6 allergen reactions and in the healthy dogs 3 and 0 allergen reactions were identified by each test, respectively. A significant difference was found between FA and healthy dogs in terms of positive allergen detection by the lymphocyte proliferation test, suggesting that the test can be useful to differentiate FA from healthy dogs but not from CAD. Both tests were repeated in 6 of the dogs with FA after a 1.5- to 5-month elimination diet trial. The IgE concentrations in 9 of 11 of the positive reactions decreased by 20-80%, whereas all the positive reactions in the lymphocyte proliferation test decreased to nearly zero (P