Cryopreservation of Semen from Domestic Livestock
Cryopreservation of Semen from Domestic Livestock
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DOI：
10.1007/978-1-4939-2193-5_10
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发表时间：
2015-01-01
期刊：
影响因子：
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通讯作者：
Oldenhof, Harriette
中科院分区：
文献类型：
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作者：
Sieme, Harald;Oldenhof, Harriette
在现代家畜育种中，冷冻保存的精液通常用于人工授精。精子冷冻保存可以确保未来的繁殖，并且授精剂量可以很容易地运输。用于冷冻保存的精液的处理可以以最小的努力和相对较低的成本完成。在本章中，我们描述了种马和公牛精子的整个冷冻保存过程，包括在初级和冷冻稀释剂中稀释精子，在吸管中冷却和包装，在液氮蒸气中冷冻，低温储存和解冻。特别强调的是在两步稀释法中使用的常用的主要和冷冻稀释剂（脱脂牛奶稀释剂“INRA-82”，TRIS-蛋黄稀释剂“TEY”）的制备。此外，还描述了在冷冻过程中不同温度状态下所需的不同冷却速率。在使用专门的自动化设备和简单设备的情况下描述了冷冻保存程序。
In modern livestock breeding, cryopreserved semen is routinely used for artificial insemination. Sperm cryopreservation secures future reproduction, and insemination doses can be easily shipped. Processing of semen for cryopreservation can be done with minimal efforts and relatively low costs. In this chapter we describe the entire cryopreservation process for stallion and bull sperm including dilution of sperm in primary and freezing extender, cooling and packaging in straws, freezing in liquid nitrogen vapor, cryogenic storage, and thawing. Special emphasis is given on preparation of commonly used primary and freezing extenders (skim milk extender "INRA-82", TRIS-egg yolk extender "TEY") used in a two-step dilution approach. Furthermore the different cooling rates needed in different temperature regimes during the freezing process are being described. Cryopreservation procedures are described in case of using both specialized automated equipment and simple equipment.