PATHOLOGICAL-CHANGES IN SMALL-INTESTINE OF NEONATAL PIGS INFECTED WITH A PIG REOVIRUS-LIKE AGENT (ROTAVIRUS)
PATHOLOGICAL-CHANGES IN SMALL-INTESTINE OF NEONATAL PIGS INFECTED WITH A PIG REOVIRUS-LIKE AGENT (ROTAVIRUS)
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DOI：
10.1016/0021-9975(77)90026-3
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发表时间：
1977-01-01
影响因子：
0.8
通讯作者：
MCNULTY, MS
中科院分区：
文献类型：
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作者：
PEARSON, GR;MCNULTY, MS
初乳剥夺仔猪感染猪轮状病毒后消化道病理改变。在感染后13 ~ 168 h的时间间隔内，分别杀死14头感染仔猪和7头对照仔猪。免疫荧光染色显示，24 ~ 96 h的小肠绒毛上皮细胞中存在病毒抗原，48 ~ 97 h的绒毛上荧光细胞较少。在168 h p.i.的粪便中可见荧光细胞。对小肠从幽门到回肠末端的10个部位进行了组织病理学检查，并测量了十二指肠、中小肠和回肠末端的绒毛长度、隐窝深度和有丝分裂指数。与病毒抗原存在相关的病理改变局限于小肠。回肠末端的绒毛长度在24 h时开始减少，在48-168 h时，小肠中部和回肠末端的绒毛出现了明显的发育迟缓。绒毛的发育不良和粘连导致扁平的绒毛状粘膜，但整个小肠的病变并不均匀。
Pathological changes in the alimentary tract of colostrum-deprived piglets infected with a pig rotavirus are described. Fourteen infected and 7 control piglets were killed at intervals over the period from 13 h post infection (p.i.) to 168 h p.i. Immunofluorescent staining showed that viral antigen was present in villous epithelial cells of the small intestine from 24-96 h p.i. Although fewer fluorescing cells were seen from 48-97 h p.i. on the villi, fluorescing cells were demonstrated in feces up to 168 h p.i. Histopathological examination was carried out on 10 sites in the small intestine from the pylorus to the terminal ileum and villus length, crypt depth and mitotic index were measured in the duodenum, middle small intestine and terminal ileum. Pathological changes associated with the presence of viral antigen were confined to the small intestine. A reduction in villus length was first observed in the terminal ileum at 24 h p.i., and from 48-168 h p.i. marked stunting of villi in the middle small intestine and terminal ileum occurred. Stunting and adhesion of the villi resulted in a flat, avillous mucosa, but the lesions were not uniform throughtout the small intestine.