Rapidly serum‐degradable hydrogel templating fabrication of spherical tissues and curved tubular structures
Rapidly serum‐degradable hydrogel templating fabrication of spherical tissues and curved tubular structures
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DOI：
10.1002/bit.24550
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发表时间：
2012-11
影响因子：
3.8
通讯作者：
S. Sakai;Hitomi Inagaki;Yang Liu;Tomohiro Matsuyama;T. Kihara;J. Miyake;K. Kawakami;M. Taya
中科院分区：
文献类型：
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作者：
S. Sakai;Hitomi Inagaki;Yang Liu;Tomohiro Matsuyama;T. Kihara;J. Miyake;K. Kawakami;M. Taya
三维组织制造技术的发展仍然是组织工程面临的重大挑战。我们使用从苯酚取代支链淀粉（AP‐Ph）中获得的水凝胶作为模板，在I型胶原凝胶中制备多细胞球形组织（MSTs）和内皮化的弯曲管状结构。直径200µm的AP - Ph水凝胶微粒和直径500µm的纤维在含血清培养基中浸泡数小时内消失。将HeLa细胞和人内皮细胞分别包裹在微颗粒和水凝胶纤维中，然后包埋在海藻酸钙微胶囊或胶原凝胶中。封闭的细胞在含血清的培养基中被释放到水凝胶降解形成的空腔中。释放的HeLa细胞在球形空腔中生长并形成mst，最终填充空腔。通过柠檬酸钠螯合使海藻酸钙水凝胶微胶囊膜液化，可以很容易地收获球形组织。释放的内皮细胞生长在管腔表面，形成管状结构。细胞迁移到胶原凝胶中形成内皮细胞网络。这些结果证明了血清可降解的AP - Ph水凝胶在构建三维组织方面的潜力。Biotechnol。Bioeng。2012;109: 2911 - 2919。©2012 Wiley期刊公司
Development of the techniques for fabricating three‐dimensional tissues still poses significant challenges for tissue engineering. We used hydrogels obtained from phenol‐substituted amylopectin (AP‐Ph) as templates for preparing multicellular spherical tissues (MSTs) and endothelialized curved tubular structures in type I collagen gel. AP‐Ph hydrogel microparticles of diameter 200 µm and fibers of diameter 500 µm disappeared within hours of soaking in a serum‐containing medium. HeLa cells and human endothelial cells were enclosed in the microparticles and hydrogel fibers, respectively, and then embedded in Ca‐alginate microcapsules or the collagen gel. The enclosed cells were released in cavities formed by hydrogel degradation in the serum‐containing medium. The released HeLa cells in the spherical cavities grew and formed MSTs, eventually filling the cavities. The spherical tissues were easily harvested by liquefying the Ca‐alginate hydrogel microcapsule membrane by chelation using sodium citrate. The released endothelial cells grew on the tubular cavity surfaces and formed tubular structures. An endothelial cell network was formed by cell migration into the collagen gel. These results demonstrate the potential of serum‐degradable AP‐Ph hydrogels in constructing three‐dimensional tissues. Biotechnol. Bioeng. 2012; 109: 2911–2919. © 2012 Wiley Periodicals, Inc.