Silencing of miR-1-1 and miR-133a-2 cluster expression by DNA hypermethylation in colorectal cancer
Silencing of miR-1-1 and miR-133a-2 cluster expression by DNA hypermethylation in colorectal cancer
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DOI：
10.3892/or.2012.1899
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发表时间：
2012-09-01
期刊：
影响因子：
4.2
通讯作者：
Tsai, Kuo-Wang
中科院分区：
文献类型：
--
作者：
Chen, Wei-Shone;Leung, Chuang-Man;Tsai, Kuo-Wang
microRNA是小型非编码RNA分子，在大肠癌（CRC）发育的多步过程中起重要作用。本研究评估了miR-1-1与miR-133a-2表达与DNA甲基化之间的关系，以及其在CRC中的假定生物学作用。结果表明，DNA甲基化调节CRC细胞系中miR-1-1和miR-133a-2簇的表达。使用Stem-Loop实时聚合酶链反应，在64个成对的组织样品（CRC肿瘤和相邻的正常粘膜）中进一步评估了miR-1和miR-133a的表达。与邻近的正常粘膜相比
MicroRNAs are small non-coding RNA molecules that play important roles in the multistep process of colorectal carcinoma (CRC) development. The present study evaluated the relationship between miR-1-1 and miR-133a-2 expression and DNA methylation, and its putative biological role in CRC. The results indicated that DNA methylation regulated the expression of the miR-1-1 and miR-133a-2 cluster in CRC cell lines. Expression of miR-1 and miR-133a was further evaluated in 64 paired tissue samples (CRC tumor and adjacent normal mucosa) using the stem-loop real-time polymerase chain reaction. The miR-1-133a cluster displayed significantly lower expression in CRC tissue compared to adjacent normal mucosa (P