Evidence for soluble factors regulating cell death and cell proliferation in primary cultures of rabbit endometrial cells grown on collagen.
Evidence for soluble factors regulating cell death and cell proliferation in primary cultures of rabbit endometrial cells grown on collagen.
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在胶原蛋白上生长的兔子宫内膜细胞的原代培养物中调节细胞死亡和细胞增殖的可溶性因子的证据。
DOI：
10.1073/pnas.83.13.4784
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发表时间：
1986
影响因子：
11.1
通讯作者：
Gerschenson,LE
中科院分区：
文献类型：
--
作者：
Lynch,MP;Nawaz,S;Gerschenson,LE
原代培养的兔子宫内膜细胞生长在胶原基质上表现出周期性的变化，在整个延长的培养期的DNA含量。这些周期的特征在于培养物的DNA含量或存在的细胞数量的显著增加和减少的时期，但在整个培养期间，总DNA没有净变化。细胞增殖和细胞死亡的速率随着培养时间的推移而变化，其周期性与DNA的变化相同。无论是细胞增殖率的变化还是细胞死亡率的变化都不足以解释DNA的变化。相反，在细胞增殖和细胞死亡之间似乎存在反馈机制，使得当一个增加时，另一个伴随增加，以维持总培养物质量的稳态。这种稳态似乎是由细胞产生的可溶性细胞增殖因子（CPF）和细胞死亡因子（CDF）介导的。CPF和CDF可以从条件培养基或培养的细胞提取物中获得。这些生物活性是热和胰蛋白酶敏感的。这些培养物中细胞死亡的主要模式似乎是细胞凋亡或程序性细胞死亡，其特征是更新上皮。这些数据表明，该组织培养模型系统代表了一个更新的细胞群，其中包含干细胞及其后代，其总生长受到CPF和CDF的严格调控。因此，它提供了一个模型系统，在其中研究内稳态，以及它如何在增生和瘤形成中改变，以及激素对其的调节。
Primary cultures of rabbit endometrial cells grown on collagen substrates exhibit cyclic changes in DNA content throughout extended periods of culture. These cycles are characterized by periods of significant increases and decreases in the DNA content of the cultures or number of cells present, yet through the entire duration of culture there is no net change in the total DNA. The rates of cell proliferation and cell death change through time in culture with the same periodicity as the changes in DNA. Neither changes in the rate of cell proliferation nor the rate of cell death alone are sufficient to account for the changes in DNA. Rather, there appears to be a feedback mechanism operating between cell proliferation and cell death such that when one increases, the other increases concomitantly in order to maintain a homeostasis in total culture mass. This homeostasis appears to be mediated by a soluble cell proliferation factor (CPF) and a cell death factor (CDF) produced by the cells. CPF and CDF may be obtained from either conditioned media or cultured cell extracts. These biological activities are heat and trypsin sensitive. The major mode of cell death in these cultures appears to be apoptosis or programmed cell death, characteristic of renewing epithelia. The data suggest that this tissue culture model system represents a renewing cell population containing stem cells and their progeny, whose total growth is strictly regulated by CPF and CDF. As such, it provides a model system in which to study homeostasis and how it may be altered in hyperplasia and neoplasia, as well as its regulation by hormones.