Homogeneous catalytic deuteration of fatty acyl chains as a tool to detect lipid phase transitions in specific membrane domains: a Fourier Transform Infrared spectroscopic study.
Homogeneous catalytic deuteration of fatty acyl chains as a tool to detect lipid phase transitions in specific membrane domains: a Fourier Transform Infrared spectroscopic study.
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脂肪酰基链的均相催化氘化作为检测特定膜域中脂质相变的工具：傅里叶变换红外光谱研究。
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发表时间：
1993
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影响因子：
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通讯作者：
L. Vigh
中科院分区：
文献类型：
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作者：
Tony Alan Ratliff;B. Szalontai;F. Joó;C. Wistrom;L. Vigh
用水溶性催化剂和氘气对合成磷脂分子进行了氘化。傅里叶变换红外光谱可以同时监测氘化段和未受影响的脂质分子体部分的物理状态。结果表明，在多层磷脂体系中，氘化段可以用作结构探针。而nu（C-H）频率代表了所有存在的烷基链的平均构象顺序，通过跟踪nu（C-D）振动的变化，可以估计在烃核中特定深度标记的膜域氘的迁移率。讨论了这种新方法在生物膜研究中的潜在重要性。
Synthetic phospholipid molecules have been deuterated by using a water soluble catalyst and deuterium gas. The physical state of both deuterated segments and unaffected bulk part of the lipid molecules can be monitored simultaneously by Fourier Transform Infrared Spectroscopy. It is shown on multilamellar phospholipid systems that the deuterated segments can be used as structural probes. Whereas the nu(C-H) frequencies represent an average conformational order along all the alkyl chains present, by following changes in nu(C-D) vibrations, mobility of those membrane domains deuterium labeled at specific depths in the hydrocarbon core can be estimated. The potential importance of this new approach in the study of biological membranes is discussed.