Differential Expression Profiles of microRNAs in NIH3T3 Cells in Response to UVB Irradiation
Differential Expression Profiles of microRNAs in NIH3T3 Cells in Response to UVB Irradiation
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DOI：
10.1111/j.1751-1097.2008.00482.x
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发表时间：
2009-05-01
影响因子：
3.3
通讯作者：
Ding, Zhen-Hua
中科院分区：
文献类型：
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作者：
Guo, Ling;Huang, Zhong-Xi;Ding, Zhen-Hua
MicroRNA（miRNAs）是一类小分子非编码RNA，在动植物的发育、细胞增殖和分化等生物学过程中发挥重要作用。在这里，我们报告的差异表达谱的miRNAs和特征推定的靶基因在NIH 3 T3细胞在一系列不同的时间点后UVB照射（与没有UVB照射相比）。通过miRNA芯片技术检测成熟miRNA基因的相对表达量，并通过实时定量逆转录聚合酶链反应（qRT-PCR）进行验证。用GOstat软件（http：//www.example.com）将这些miRNAs的潜在靶基因分为不同的功能类别。gostat.wehi.edu.au本研究中的几种miRNA在不同时间点高度表达，尤其是mmu-miR-365和mmu-miR-21。3种miRNAs低表达，其中mmu-miR-465在所有时间点均显示低水平表达，而50 J m（-2）UVB照射后mmu-miR-296和mmu-miR-376 c分别在6和12 h显示低水平表达。我们的研究为紫外线调控的miRNA表达的全局表征提供了基础。
MicroRNAs (miRNAs) are known as a kind of small, noncoding RNA, which play an important role in mediating many biological processes such as development, cell proliferation and differentiation in plants and animals. Here we report the differential expression profiles of miRNAs and characterized putative target genes in NIH3T3 cells at a series of different time points after UVB irradiation (compared with no UVB irradiation). The relative expression of mature miRNA genes was determined by miRNA microarray technique and the results were confirmed by real-time reverse transcriptase polymerase chain reaction (qRT-PCR). Potential target genes of these miRNAs were classified into different function categories with the GOstat software (http://gostat.wehi.edu.au). Several miRNAs in this study expressed highly at different time points, especially mmu-miR-365 and mmu-miR-21. Three miRNAs were lowly expressed, of which mmu-miR-465 showed low levels of expression at all time points, whereas after 50 J m(-2) UVB irradiation mmu-miR-296 and mmu-miR-376c showed low levels of expression at 6 and 12 h, respectively. Our study provided a basis for the global characterization of UV-regulated miRNA expression.