The Fate of Major Royal Jelly Proteins during Proteolytic Digestion in the Human Gastrointestinal Tract.
The Fate of Major Royal Jelly Proteins during Proteolytic Digestion in the Human Gastrointestinal Tract.
复制标题
主要蜂王浆蛋白在人类胃肠道蛋白水解消化过程中的命运
DOI：
10.1021/acs.jafc.8b00961
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发表时间：
2018
影响因子：
6.1
通讯作者：
A Buttstedt
中科院分区：
文献类型：
--
作者：
CI Mureşan;A Schierhorn;A Buttstedt
皇家浆（RJ）是具有复杂组成的蜂箱产品，主要的皇家浆蛋白（MRJPs）是最丰富的蛋白质。细胞培养和动物研究表明全长/天然MRJP具有多种生物活性。在蜂疗领域，人们认为MRJP可以对人类健康产生积极影响。然而，无论何时口服RJ，胃肠道中同化的可用性是MRJP对人类产生任何影响的先决条件。我们在这里表明，MRJPs在胃蛋白酶消化的抗性方面有所不同，MRJP 2最稳定，消化24小时后仍以全长蛋白存在。在肠阶段，使用胰蛋白酶和胰凝乳蛋白酶，MRJP被MRJP 2快速消化，再次显示出最长的稳定性（40分钟），这表明MRJP可以作为全长蛋白质到达小肠，但是如果全长蛋白质要实现任何生物活性，则必须快速再吸收。
Royal jelly (RJ) is a beehive product with a complex composition, major royal jelly proteins (MRJPs) being the most abundant proteins. Cell culture and animal studies suggest various biological activities for the full-length/native MRJPs. In the field of apitherapy, it is assumed that MRJPs can positively affect human health. However, whenever RJ is administered orally, the availability for assimilation in the gastrointestinal tract is a prerequisite for MRJPs to have any effect on humans. We here show that MRJPs vary in resistance to pepsin digestion with MRJP2 being most stable and still present as full-length protein after 24 h of digestion. In the intestinal phase, using trypsin and chymotrypsin, MRJPs are rapidly digested with MRJP2 again showing longest stability (40 min), suggesting that MRJPs can reach the small intestine as full-length proteins but then have to be resorbed quickly if full-length proteins are to fulfill any biological activity.