Prostaglandin E release from human monocytes treated with lipopolysaccharides isolated from Bacteroides intermedius and Salmonella typhimurium: potentiation by gamma interferon.
Prostaglandin E release from human monocytes treated with lipopolysaccharides isolated from Bacteroides intermedius and Salmonella typhimurium: potentiation by gamma interferon.
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用从中间拟杆菌和鼠伤寒沙门氏菌分离的脂多糖处理的人单核细胞释放前列腺素 E：γ 干扰素增强。
DOI：
10.1128/iai.59.1.398-406.1991
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发表时间：
1991
影响因子：
3.1
通讯作者：
Payne,JB
中科院分区：
文献类型：
--
作者：
Nichols,FC;Peluso,JF;Tempro,PJ;Garrison,SW;Payne,JB
本研究的目的是通过从中间拟杆菌和鼠伤寒沙门氏菌中分离出的苯酚水萃取（未分离）和高纯度LPS制剂，研究γ干扰素对人单核细胞脂多糖（LPS）反应的增强作用。通过Sepharose-CL-4B层析进一步纯化这些细菌的酚水提取LPS制剂。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、定量测定羟基脂肪酸和2-酮-3-脱氧辛烷酸含量、蛋白质污染和蒽酮反应物质来评估池柱组分中LPS的富集程度。在有和没有干扰素治疗的情况下，评估LPS对单核细胞的刺激，以前列腺素E （PGE）释放来衡量。细胞要么同时用干扰素和LPS处理，要么在LPS刺激之前用干扰素预处理。在0- 24小时和24- 48小时培养间隔期间，定量测定逆流分离单核细胞的PGE释放。与本实验室之前的结果相反，从中间芽孢杆菌和鼠伤寒沙门氏菌中提取的酚水提取的LPS制剂在刺激单核细胞释放PGE的能力方面是相似的。分子筛色谱法仅能从中间芽孢杆菌LPS中去除大量的高分子量多糖污染物，但对中间芽孢杆菌和鼠伤寒沙门氏菌LPS的效力没有显著影响。γ干扰素共处理没有增强PGE的释放与任何LPS制剂测试。然而，用γ干扰素预处理单核细胞24小时，然后暴露于LPS 24小时，导致PGE释放比单独LPS显著增强。此外，在γ干扰素预处理后，中间芽孢杆菌制剂的效力比从鼠伤寒沙门氏菌中分离的类似制备的LPS菌株强约三倍。这些结果表明γ干扰素可以选择性地增强中间芽孢杆菌LPS对人单核细胞分离株的影响。
The purpose of this investigation was to examine gamma interferon potentiation of lipopolysaccharide (LPS) responses in human monocytes by using phenol-water-extracted (unfractionated) and highly purified LPS preparations isolated from Bacteroides intermedius and Salmonella typhimurium. Phenol-water-extracted LPS preparations from these bacteria were further purified by chromatography over Sepharose-CL-4B. LPS enrichment in pooled column fractions was assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and quantitation of hydroxy-fatty acid and 2-keto-3-deoxyoctulosonic acid content, protein contamination, and anthrone-reactive material. Monocyte stimulation by LPS, measured as prostaglandin E (PGE) release, was assessed with and without gamma interferon treatment. Cells were either treated simultaneously with gamma interferon and LPS or pretreated with gamma interferon prior to LPS stimulation. PGE release from counterflow-isolated monocytes was quantitated during the 0- to 24-h and 24- to 48-h culture intervals. Contrary to previous results from this laboratory, phenol-water-extracted LPS preparations from B. intermedius and S. typhimurium were similar in their capacities to stimulate PGE release from monocytes. Molecular sieve chromatography was found to remove substantial amounts of high-molecular-weight polysaccharide contaminants only from the B. intermedius LPS but did not significantly alter the potency of either B. intermedius or S. typhimurium LPS. Gamma interferon cotreatment did not potentiate the release of PGE with any of the LPS preparations tested. However, 24-h pretreatment of monocytes with gamma interferon followed by a 24-h exposure to LPS resulted in significant potentiation of PGE release over LPS alone. In addition, B. intermedium preparations were approximately threefold more potent than similarly prepared LPS isolates from S. typhimurium following gamma interferon pretreatment. These results indicate that gamma interferon can selectively potentiate the effects of B. intermedius LPS in human monocyte isolates.