Biolaser Endothelial Trauma as a Means of Quantifying Platelet Activity in vivo
Biolaser Endothelial Trauma as a Means of Quantifying Platelet Activity in vivo
复制标题
生物激光内皮损伤作为体内量化血小板活性的一种手段
作者：
K. Arfors;D. Dhall;J. Engeset;H. Hint;N. Matheson;O. Tangen
尽管已经描述了几种体外研究血小板行为的方法，但还没有一种简单而精确的定量研究技术。有用的测量结果已经通过计数血小板微栓子形成的反应，造成的损伤，用眼钳捏暴露的兔软脑膜血管1。该方法已用于声称影响血小板粘附性的药物的初步研究2，3。然而，创伤的程度无法重复控制，此外，动物必须麻醉。在实验前暴露软脑膜血管期间造成创伤，之后必须杀死动物。一种更精确的方法是将微电极应用于仓鼠颊囊内的血管壁，并测量诱导血小板血栓所需的最小刺激（mA）4，5。但是，微电极的可重复应用是困难的，并且与软脑膜血管方法一样，动物被麻醉，经受初步创伤，然后被杀死。
ALTHOUGH several methods of studying platelet behaviourin vitrohave been described, there has not been a simple and precise technique for making quantitative studies. Useful measurements have been obtained by counting the platelet microemboli formed in response to injury caused by pinching exposed rabbit pial vessels with ophthalmic forceps1. This method has been used in preliminary studies of drugs claimed to affect platelet adhesiveness2,3. The degree of trauma, however, cannot be reproducibly controlled and, in addition, the animals must be anaesthetized. Trauma is inflicted during exposure of the pial vessels before the experiment, and afterwards the animal has to be killed. A more precise method involves the use of a microelectrode applied to the wall of a blood vessel in the cheek pouch of the hamster and the measurement in mA of the minimal stimulus required to induce a platelet thrombus4,5. But reproducible application of the microelectrode is difficult and as with the pial vessel method the animals are anaesthetized, subjected to preliminary trauma and later killed.