The clinical significance of CCBE1 expression in human colorectal cancer
The clinical significance of CCBE1 expression in human colorectal cancer
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DOI：
10.2147/cmar.s181770
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发表时间：
2018-01-01
影响因子：
3.3
通讯作者：
Yang, Chun-Guang
中科院分区：
文献类型：
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作者：
Zhao, Yan-Rong;Liu, Hao;Yang, Chun-Guang
目的：筛选和发现结直肠癌（CRC）的预后标志物具有重要的临床意义。CCBE 1在多种肿瘤中表达，其表达与淋巴管生成和血管生成相关。然而，CCBE 1表达和CRC结果之间的关联尚未报道。本研究的目的是探讨CCBEI表达的临床意义在CRC.Patients和方法：CCBEI的表达在30对新鲜的CRC组织，并与相邻的正常（AN）组织进行比较，采用定量实时PCR（qRT-PCR），蛋白质印迹和免疫组化（IHC）染色。采用组织芯片免疫组化染色方法，检测2002年1月至2007年12月间204例结直肠癌组织中CCBE 1的表达特征，分析CCBE 1与结直肠癌临床病理特征及预后的关系。CCBE 1的高表达与大肠癌的分化程度、淋巴结转移、血管浸润、肝转移及TNM分期有关（P
Purpose: The identification and discovery of prognostic markers for colorectal cancer (CRC) are of great clinical significance. CCBE1 is expressed in various tumors and its expression correlates with lymphangiogenesis and angiogenesis. However, the association between CCBE1 expression and CRC outcome has not been reported. The aim of this study was to investigate clinical significance of CCBEI expression in CRC.Patients and methods: CCBEI expression was examined in 30 pairs of fresh CRC tissues and compared with adjacent normal (AN) tissues using quantitative real-time PCR (qRT-PCR), Western blotting and immunohistochemistry (IHC) staining. Tissue microarray immunohistochemical staining was used to study the CCBEI expression characteristics of 204 CRC patient samples collected from January 2002 to December 2007, and the relationship of CCBE1 with clinicopathological features and prognosis of CRC was analyzed.Results: CCBE1 was highly expressed in CRC tissues compared with matched AN tissues (P=0.001). Moreover, high expression of CCBE1 was significantly associated with tumor differentiation, lymph node metastasis, vascular invasion, liver metastasis and TNM stage in CRC patients (P