De novo transcriptome profiling of highly purified human lymphocytes primary cells.
De novo transcriptome profiling of highly purified human lymphocytes primary cells.
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DOI：
10.1038/sdata.2015.51
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发表时间：
2015
期刊：
影响因子：
9.8
通讯作者：
Pagani M
中科院分区：
文献类型：
--
作者：
Bonnal RJ;Ranzani V;Arrigoni A;Curti S;Panzeri I;Gruarin P;Abrignani S;Rossetti G;Pagani M
为了更好地理解长链非编码RNA在人体免疫系统中的作用，我们最近使用来自13个T淋巴细胞亚群（CD4+ naive、CD4+ TH1、CD4+ TH2、CD4+ TH17、CD4+ Treg、CD4+ TCM、CD4+ TEM、CD8+ TCM、CD8+ TEM、CD8+ naive）和B淋巴细胞（B naive、B memory、B CD5+）的63个RNA样本生成了一个全面的RNA序列数据集。除CD8+ TCM和B CD5+群体有4个重复外，每个亚群有5个生物重复。RNA-Seq数据由Illumina hisansq测序仪生成，使用TruSeq v3 Cluster试剂盒。对21.92亿对末端reads （2×100 bp）进行测序，筛选后共绘制了约17亿对reads。使用不同的从头转录组重建技术鉴定了500多个以前未知的lincrna。目前的数据集可以用来驱动lincrna的功能表征，鉴定与人类免疫系统特定细胞亚群相关的新基因和调控网络。
To help better understand the role of long noncoding RNAs in the human immune system, we recently generated a comprehensive RNA-seq data set using 63 RNA samples from 13 subsets of T (CD4+ naive, CD4+ TH1, CD4+ TH2, CD4+ TH17, CD4+ Treg, CD4+ TCM, CD4+ TEM, CD8+ TCM, CD8+ TEM, CD8+ naive) and B (B naive, B memory, B CD5+) lymphocytes. There were five biological replicates for each subset except for CD8+ TCM and B CD5+ populations that included 4 replicates. RNA-Seq data were generated by an Illumina HiScanSQ sequencer using the TruSeq v3 Cluster kit. 2.192 billion of paired-ends reads, 2×100 bp, were sequenced and after filtering a total of about 1.7 billion reads were mapped. Using different de novo transcriptome reconstruction techniques over 500 previously unknown lincRNAs were identified. The current data set could be exploited to drive the functional characterization of lincRNAs, identify novel genes and regulatory networks associated with specific cells subsets of the human immune system.