A newly designed experimental system for exposure of mammalian cells to extremely low frequency magnetic fields.
A newly designed experimental system for exposure of mammalian cells to extremely low frequency magnetic fields.
复制标题
一种新设计的实验系统，用于将哺乳动物细胞暴露于极低频磁场中。
DOI：
--
复制
发表时间：
1994
影响因子：
2
通讯作者：
Hiraku Takebe
中科院分区：
文献类型：
--
作者：
J. Miyakoshi;S. Ohtsu;Junko Tatsumi;Hiraku Takebe
为了研究极低频磁场（ELFMF）的生物学效应，我们设计并制造了一种新的细胞长期高密度暴露于ELFMF的装置。ELFMF暴露系统由内置CO2培养箱的磁体发生器、交流（AC）电源、气体压缩机和培养箱温控器以及磁体冷却装置组成。将丙烯酸树脂制成的CO2培养箱插入硅钢片芯的内部空间。在该系统中，培养箱的温度保持在37 +/- 0.5 ℃。在50 Hz、556 Arms（rms;均方根）的电流下，培养箱暴露区域的最大磁通密度为500 mT（T;特斯拉）。400 mT ELFMF的长期（长达120小时）暴露不影响HL 60 RG和CCRF-CEM细胞的生长。400 mT ELFMF照射后2小时的X射线照射暴露也没有影响来自正常人和共济失调毛细血管扩张症患者的GM 0637和TAT 2SF细胞的辐射敏感性。
To examine the biological effects of extremely low frequency magnetic field (ELFMF), we have designed and manufactured a new equipment for long-term and high-density exposure of cells to ELFMF. The ELFMF exposure system consists of a generator of magnets with a built-in CO2 incubator, an alternating current (AC) power supply, a gas compressor and a thermocontroller for the incubator, and a cooling unit for the magnets. The CO2 incubator made of acrylic resin is inserted into the inner-space of the silicon steel strip-cores. In this system, the temperature of the incubator is maintained at 37 +/- 0.5 degrees C. The maximum magnetic flux density on the exposure area of the incubator is 500 mT (T; tesla) at a current of 556 Arms (rms; root mean square) at 50 Hz. The long-term (up to 120 hr) exposure of 400 mT ELFMF did not affect the growth of both HL60RG and CCRF-CEM cells originated from human leukemia. The post-X-irradiation exposure of 400 mT ELFMF for 2 hr also did not affect the radiation sensitivity of GM0637 and TAT2SF cells originated from a normal human and an ataxia telangiectasia patient.