ISOLATION OF EXFOLIATIVE TOXIN FROM STAPHYLOCOCCUS-HYICUS SUBSP HYICUS AND ITS EXFOLIATIVE ACTIVITY IN THE PIGLET
ISOLATION OF EXFOLIATIVE TOXIN FROM STAPHYLOCOCCUS-HYICUS SUBSP HYICUS AND ITS EXFOLIATIVE ACTIVITY IN THE PIGLET
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DOI：
10.1016/0378-1135(91)90153-7
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发表时间：
1991-05-01
影响因子：
3.3
通讯作者：
SAITO, H
中科院分区：
文献类型：
--
作者：
SATO, H;TANABE, T;SAITO, H
从葡萄球菌培养24 h的无菌滤液中分离出剥脱毒素。菌种P-1。采用50-80%饱和硫酸铵沉淀、Sephadex G-75柱凝胶过滤、deae -纤维素柱层析等方法对S. hyicus （shET）产剥脱毒素进行部分纯化。部分纯化的薄片（pp-薄片）在仔猪皮内或皮下注射后8至12小时引起脱落。然而，热处理的pp- sheet在注射后24小时内不会引起仔猪脱落。注射pp- sheet后12小时的皮肤组织病理学检查显示，角质层和颗粒层之间以及颗粒层处表皮内有一个卵裂面。
Exfoliative toxin was isolated from the sterile cell-free filtrate of 24 h culture of Staphylococcus hyicus subsp. hyicus strain P-1. The partial purification of exfoliative toxin produced by S. hyicus (shET) was performed by precipitation with 50-80% saturated ammonium sulfate, gel filtration on a Sephadex G-75 column and column chromatography on DEAE-cellulose. Partially purified shET (pp-shET) caused exfoliation in piglets at 8 to 12 h after intradermal or subcutaneous injection. However, heat-treated pp-shET did not cause exfoliation in piglets for up to 24 h after injection. On histopathological examination of the skin at 12 h after injection of pp-shET, an intraepidermal cleavage plane was shown between the stratum corneum and stratum granulosum and at the stratum granulosum.