The testicular and epididymal luminal amino acid microenvironment in the rat.
The testicular and epididymal luminal amino acid microenvironment in the rat.
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DOI：
10.1002/j.1939-4640.1990.tb00186.x
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发表时间：
1990-11
影响因子：
--
通讯作者：
Barry T. Hinton
中科院分区：
文献类型：
--
作者：
Barry T. Hinton
在动脉和睾丸静脉血中，以及在生精小管、睾丸网、附睾头、附睾体和附睾尾的液体中测定氨基酸浓度。动脉血和睾丸静脉血之间的任何氨基酸的浓度没有显着差异，而动脉/静脉血和睾丸间质液之间有显着差异。在生精小管液（STF）和睾丸网液（RTF）中测得的主要氨基酸为甘氨酸、丙氨酸、谷氨酸和谷氨酰胺。RTF含有大致相等浓度的碱性和总氨基酸，但17倍高的酸性氨基酸和1.2和1.3倍低的不带电的极性和非极性氨基酸，分别与STF相比。头液中总氨基酸浓度达到50 nmol/L以上，但随后下降到头体和尾体的50%和0.1%左右。附睾腔液中测得的主要氨基酸是谷氨酸和牛磺酸;谷氨酸占头液中测得的总氨基酸的约90%。谷氨酸盐和牛磺酸的存在下，附睾腔主要是由于直接贡献，从附睾上皮，测量使用分滴停流微灌注技术。管腔内的其他几种氨基酸也来源于附睾上皮。氨基酸分别占头、体和尾液总渗透压的约20%、9%和2%。（250字处删节）
Concentrations of amino acids were measured in arterial and testicular venous blood, and in fluids from the seminiferous tubule, rete testis, and the caput, corpus, and cauda epididymidis. There were no significant differences in the concentrations of any amino acids between arterial and testicular venous blood, whereas there were significant differences between arterial/venous blood and testicular interstitial fluid. The predominant amino acids measured within seminiferous tubule fluid (STF) and rete testis fluid (RTF) were glycine, alanine, glutamate, and glutamine. RTF contained approximately equal concentrations of basic and total amino acids, but 17 times higher acidic amino acids and 1.2 and 1.3 times lower uncharged polar and nonpolar amino acids, respectively, compared to STF. The concentration of total amino acids within caput fluid reached over 50 nmol/L, but then declined to approximately 50% and 0.1% of caput for corpus and cauda, respectively. The predominant amino acids measured within epididymal luminal fluids were glutamate and taurine; glutamate contributed to approximately 90% of the total amino acids measured in caput fluid. The presence of glutamate and taurine within the epididymal lumen is due primarily to a direct contribution from the epididymal epithelium, as measured using the split-drop stopped-flow microperfusion technique. Several other amino acids within the lumen also originate from the epididymal epithelium. Amino acids contribute approximately 20%, 9%, and 2% of the total osmolality of caput, corpus, and cauda fluid, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)