Chronic psychosocial stress in male mice causes an up-regulation of scavenger receptor class B type 1 protein in the adrenal glands
Chronic psychosocial stress in male mice causes an up-regulation of scavenger receptor class B type 1 protein in the adrenal glands
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DOI：
10.3109/10253890.2013.793303
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发表时间：
2013-07-01
影响因子：
2.3
通讯作者：
Reber, Stefan O.
中科院分区：
文献类型：
--
作者：
Fuechsl, Andrea M.;Uschold-Schmidt, Nicole;Reber, Stefan O.
暴露于慢性从属群体住房（CSC， 19天）的小鼠对急性异型应激源（升高平台（EPF）， 5分钟）表现出夸张的肾上腺皮质酮反应，尽管在促肾上腺皮质激素（ACTH）分泌方面与暴露于EPF的单房对照（SHC）小鼠没有差异。在本研究中，我们提出了csc诱导的肾上腺产生和分泌皮质酮能力的增加是否与肾上腺可利用性和/或皮质酮前体分子胆固醇的动员能力增强相平行的问题。通过油红染色和western blot分析，我们发现CSC和SHC小鼠皮质脂滴的相对密度以及激素敏感脂肪酶、3-羟基-3-甲基戊二酰辅酶A （HMG-CoA）还原酶和低密度脂蛋白受体（LDL-R）的相对蛋白表达量相当。然而，在CSC暴露后，清道夫受体B类1型（SR-BI）的相对蛋白表达增加。此外，血浆高密度脂蛋白胆固醇（HDL-C）和低密度脂蛋白胆固醇（LDL-C）分析显示CSC小鼠的LDL-C水平升高。再加上肾上腺重量的显著增加，显然是由肾上腺皮质细胞增生介导的，这些数据强烈表明，慢性社会心理应激小鼠的肾上腺可利用性和动员胆固醇的能力增强，有助于它们在急性异型应激源暴露期间增加体内皮质酮反应。
Mice exposed to chronic subordinate colony housing (CSC, 19 days) show an exaggerated adrenal corticosterone response to an acute heterotypic stressor (elevated platform (EPF), 5 min) despite no difference from EPF-exposed single-housed control (SHC) mice in corticotropin (ACTH) secretion. In the present study, we asked the question whether this CSC-induced increase in adrenal capability to produce and secrete corticosterone is paralleled by an enhanced adrenal availability and/or mobilization capacity of the corticosterone precursor molecule cholesterol. Employing oil-red staining and western blot analysis we revealed comparable relative density of cortical lipid droplets and relative protein expression of hormone-sensitive lipase, 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase and low-density lipoprotein receptor (LDL-R) between CSC and SHC mice. However, relative protein expression of the scavenger receptor class B type 1 (SR-BI) was increased following CSC exposure. Moreover, analysis of plasma high-density lipoprotein-cholesterol (HDL-C) and LDL-cholesterol (LDL-C) revealed increased LDL-C levels in CSC mice. Together with the pronounced increase in adrenal weight, evidently mediated by hyperplasia of adrenocortical cells, these data strongly indicate an enhanced adrenal availability of and capacity to mobilize cholesterol in chronic psychosocially-stressed mice, contributing to their increased in vivo corticosterone response during acute heterotypic stressor exposure.